In Japan, forensic examination of cannabis seeds requires proof “being cannabis” and germinable”, is currently conducted by continuing cultivation the for several weeks after germination tests performing morphological observations chemical analysis on true leaves. We have previously constructed a rapid simple method identification combining color reaction with DNA testing. this study, effective...

Monocytes from blood and from spleen have been cultivated in fluid medium in Carrel flasks for over 2 months. Diluted serum supplied all the essential nutritive substances. Cultivation in fluid was made possible by adjusting the initial pH of the fluid to 7.4, and not allowing it to fall below 7.0 or 6.8. The cells remained in good condition when the pH was adjusted with either lactic acid, hyd...

Use of chemical fertilizers, pesticides and sewage sludge in agriculture lands caused the increase of heavy metals concentration in the soils in many parts of the world. The purpose of this article is studying the impacts of agricultural activities on the accumulation of Cd, Co, Cr, Cu, Ni and Pb in the soil. The soil samples were taken by systematic method from agriculture fields with cultivat...

The effects of drip irrigation and different cultivation methods on seedgermination and growth of Puccinellia tenuiflora in saline-sodic soils wereinvestigated in field experiments during 2008-2010 in the Songnen Plain, China.Drip irrigation was applied to waste saline-sodic P. tenuiflora grasslands for bothflat and ridge cultivation methods. Drip irrigation affected the distributions ofelectri...

In recent years the technique for the continuous cultivation of microorganisms has been adapted for a variety of uses. Gerhart (1946) and Barnes and Dewey (1947) have reported on the advantage of continuous cultivation over conventional methods for obtaining increased yields of bacterial cells, and von Hofsten et al. (1953) have extended the method to the cultivation of fungi. Continuous cultiv...

The long-term stationary culture of postimplanatation embryos without extraembryonic membranes is a method to assess their developmental potential in vitro. The method was almost exclusively used on rat embryos, while mouse embryos were considered unsuitable due to their poor differentiation. In present study the postimplantation mouse embryos were used to verify potential of this method in mic...

Biomass yields and sporulation of Beauveria bassiana was concerned on culture conditions, environmental factors and cultivation method. We optimized the best culture conditions for biomass yields of B. bassiana IBC1201 with the novel "two-stage" cultivation method as well as orthogonal matrix method. Firstly, we cultured spore suspension on the basal medium (sucrose 19.00 g, soy peptone 4.06 g,...