The present study was carried out to detect the presence of Alfalfa mosaic alfamovirus (AMV), cucumber mosaic cucumovirus (CMV), lettuce mosaic potyvirus (LMV), cucumber green mottle mosaic virus (CGMMV), tomato bushy stunt tombusvirus (TBSV), tobacco mosaic tobamovirus (TMV), tomato black ring nepovirus (TBRV) and tomato mosaic tobamovirus (ToMV) in seeds of pepper, tomato, cucumber and lettuc...

Andalusia, southern Spain, is a major horticultural production region within the Mediterranean, where over 10,000 ha are dedicated to the production of pepper (Capsicum annuum L.). Approximately two-thirds of the area dedicated to this crop is in a greenhouse and the remaining one-third is comprised of open field crops. Using pepper as a model, we identified and compared the major diseases caus...

We previously devised a cucumber mosaic virus (CMV)-based vector system carrying microRNA target mimic sequences for analysis of microRNA function in Arabidopsis thaliana. We describe an improved version in which target mimic cloning is achieved by annealing two partly-overlapping complementary DNA oligonucleotides for insertion into an infectious clone of CMV RNA3 (LS strain) fused to the caul...

In this study, it was aimed to reveal some viruses that are a problem in Dahlia plants grown for ornamental parks and home gardens Selçuklu, Karatay Meram districts of Konya. For reason, the 2019 summer, leaf samples were taken from 152 showing symptoms similar virus disease such as mosaic, deformations, yellowing, blight on leaves stunted plants. Possible infections Cucumber mosaic (CMV), Toma...

A new cucumber mosaic virus (CMV) subgroup based on recent sequence data has been proposed by Palukaitis and Zaitlin (1997) to distinguish between some strains in the former subgroup I. The three subgroups are tentatively named IA, IB and II. Here we describe a simple and rapid procedure based on the reverse transcriptase-polymerase chain reaction (RTPCR) followed by enzymatic digestion (RFLP) ...

Natural infection of Cucumber mosaic virus (CMV) in Indian long pepper (Piper longum L.) and betel vine (Piper betle L.) was detected by reverse transcription polymerase chain reaction (RT-PCR). The coat protein gene sequences of CMV infecting these hosts were amplified. The resulting amplicons were cloned and sequenced. In both the cases coat protein gene consisted of 657 nucleotides, potentia...

The cucumovirus, cucumber mosaic virus (CMV), requires both the 3a movement protein (MP) and the capsid protein (CP) for cell-to-cell movement. Replacement of the MP of CMV with the MP of the umbravirus, groundnut rosette virus (GRV), which does not encode a CP, resulted in a hybrid virus, CMV(ORF4), which could move cell to cell in Nicotiana tabacum and long distance in N. benthamiana. After r...

This study was initiated to isolate and characterize Cucumber mosaic virus based on serological, biological, molecular approaches. Leaf samples were collected from symptomatic cucumber plants grown in protected fields at Plant protection Dept., College of Agricultural Engineering Sciences, the University Baghdad Al-Jhdryaa used for biological assays. ELISA using CMV specific commercial kit dete...

background cucumber mosaic virus (cmv) has isometric particles with a diameter of about 28–29 nm. a useful method to recognize regions from mutagenesis in the coat protein (cp) gene are important for particle assembly and the expression of viral in cp expression systems like bacteria such as e.coli or yeast as well as their assembly into virus-like particles (vlp). objectives in this paper, we ...

Genetic bottlenecks may occur in virus populations when only a few individuals are transferred horizontally from one host to another, or when a viral population moves systemically from the infection site. Genetic bottlenecks during the systemic movement of an RNA plant virus population were reported previously (H. Li and M. J. Roossinck, J. Virol. 78:10582-10587, 2004). In this study we mechani...