The molecular size and conformation of chloroplast (ct-) DNA has been studied by renaturation kinetics and electron microscopy. Ct-DNA of buoyant density 1.698 f 0.001 g per cm3 showed a T,, of 84” and a homogeneous melting pattern. Denatured and sheared c&DNA renatured as a single kinetic class with no suggestion of repeating sequences. Ct-DNA is found to have a molecular weight of 95 x lo6 by...

The structural core of DNA, a continuous stack of aromatic heterocycles, the base pairs, which extends down the helical axis, gives rise to the fascinating electronic properties of this molecule that is so critical for life. Our laboratory and others have developed diverse experimental platforms to investigate the capacity of DNA to conduct charge, termed DNA-mediated charge transport (DNA CT)....

Naturally transgenic plants represent the result of Agrobacterium-mediated gene transfer. T-DNA soil bacteria Agrobacterium integrated into genome is called cellular (cT-DNA) [1]. Today, more than 50 species naturally plants, or natural GMO (nGMO) are known [2, 3]. The function cT-DNA in remains unknown. It assumed that fixation transgenes could give different selective advantages depending on ...

Chartreusin (CT), an antibiotic produced by Strepto myceschartreusls,was recentlyfoundto be activeagainst experimental tumors B16 melanoma and L1210 and P388 leukemia. This report describes the correlation between ftsbiologicalandbiochemicaleffectson mouseL1210and P388leukemIa. CT was growthInhibitoryto L1210cells and other mam malian cells tested (drugconcentrationrequiredfor 50% inhibition of...

Damaged bases in DNA are known to lead to errors in replication and transcription, compromising the integrity of the genome. We have proposed a model where repair proteins containing redox-active [4Fe-4S] clusters utilize DNA charge transport (CT) as a first step in finding lesions. In this model, the population of sites to search is reduced by a localization of protein in the vicinity of lesio...

A new Pt(II) complex, [Pt(DIP)(LL)](NO(3))(2) (in which DIP is 4,7-diphenyl-1,10-phenanthroline and LL is the aliphatic dinitrogen ligand, N,N-dimethyl-trimethylenediamine), was synthesized and characterized using different physico-chemical methods. The interaction of this complex with calf thymus DNA (CT-DNA) was investigated by absorption, emission, circular dichroism (CD), and viscosity meas...

background: discovery of short cell free fetal dna (cffdna) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. the use of cffdna to set up noninvasive prenatal test is limited due to the low concentration of fetal dna in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. the aim of this study was to ev...

A series of novel potential DNA bis-intercalators were designed and synthesized, in which two glucuronic acids were linked by ethylenediamine, and the glucuronic acid was coupled with various chromophores, including quinoline, acridine, indole and purine, at the C-1 position. The preliminary binding properties of these compounds to calf thymus DNA (CT-DNA) have been investigated by UV-absorptio...

Abstract The syntheses of [Cu(PPh 3 ) 2 (L)]NO and (L-SO Na)]NO were achieved through the reaction Cu(PPh NO equimolar amount ligands (L = 5,6-diphenyl-3-[2-pyridyl]-1,2,4-triazine; LSO Na 5,6-diphenyl-3-[2-pyridyl]-1,2,4-triazine-4,4?-disulfonic acid disodium salt). complexes characterized by NMR IR spectroscopy mass spectrometry. compounds exhibit similar absorption emission spectra, suggesti...

Endonuclease III (EndoIII) is a base excision repair glycosylase that targets damaged pyrimidines and contains a [4Fe-4S] cluster. We have proposed a model where BER proteins that contain redox-active [4Fe-4S] clusters utilize DNA charge transport (CT) as a first step in the detection of DNA lesions. Here, several mutants of EndoIII were prepared to probe their efficiency of DNA/protein charge ...