We investigate distributions of crystal violet and malachite green on plasmonic surfaces by principal component analysis (PCA) imaging of surface-enhanced hyper-Raman scattering (SEHRS) data. As a two-photon excited Raman scattering process, SEHRS provides chemical structure information based on molecular vibrations, but follows different selection rules than the normal, one-photon excited surf...

Recent evaluations of the MacConkey agar test for differentiation of rapidly growing mycobacteria have revealed that certain strains of Mycobacterium fortuitum and M. chelonei that were expected to grow on MacConkey agar failed to do so. Investigation of two formulations of MacConkey agar showed that these two species grew better on the medium when the crystal violet dye was omitted. Several po...

We have used resonant soft x-ray scattering to study the effects of discommensuration on the hole Wigner crystal (HC) in the spin ladder Sr(14-x)CaxCu24O41 (SCCO). As the hole density is varied the HC forms only with the commensurate wave vectors L(L) = 1/5 and L(L) = 1/3; for incommensurate values it "melts." A simple scaling between L(L) and temperature is observed, tau1/3/tau1/5 = 5/3, indic...

Fig. 7. Simultaneous inhibition of PDK1 and PLCγ1 does not enhance the effect of inhibition of PDK1 or PLCγ1 alone on cell invasion. (A,B) Results from an invasion assay performed with MDA-MB-231 and A375M cells, respectively, expressing shRNA targeting PLCγ1 or a 3MUT non-targeting sequence, and treated with 1 μM GSK2334470. (C,D) Results from invasion assay performed with the indicated MDA-MB...

Several techniques have been used to quantify the cytotoxicity produced by Mycobacterium tuberculosis bacilli on cell monolayers; however, they are semi-quantitative or time consuming. Herein, a method based on crystal violet (CV) uptake by THP-1 cell monolayers is described. This colorimetric method quantifies the cytotoxic effect as a function of the number of remaining cells after the infect...

BACKGROUND P21-activated kinase 1 (Pak1) integrates various signaling pathways that are vital to cell survival and function. This study was performed to evaluate whether sex steroids may regulate the expression of Pak1 in endometrial cells as well as whether its expression is increased in the eutopic endometrium of women with endometriosis. METHODS Following in vitro estradiol (E(2)) and/or m...

Biofilms forming on the surface of biomaterials can cause intractable implant-related infections. Bacterial adherence and early biofilm formation are influenced by the type of biomaterial used and the physical characteristics of implant surface. In this in vitro research, we evaluated the ability of Staphylococcus epidermidis, the main pathogen in implant-related infections, to form biofilms on...

Green-fluorescent intracellular enteropathogenic bacteria were observed after infected HeLa cell monolayers were stained with acridine orange and counterstained with crystal violet at least 3 h after infection.

Abstract A total of 46 Staphylococcus aureus isolates from fish and ground beef were tested for the agr types, icaABCD genes, biofilm formation at 12, 25 37 °C by microtiter plate MTT assays. All positive icaABD while 97.8% icaC . produced biofilms °C, but 93.5% them also producers 12 °C. There was no significant difference in between using crystal violet assay ( P > 0.05). However, statisti...