BACKGROUND The objective of this study was to investigate the effect of vitrification and in vitro maturation on the mitochondrial distribution and ATP content of oocytes. METHODS The oocytes at Germinal Vesicle (GV) and Metaphase II (MII) stages were recovered from 6-8 week old NMRI strain female mice. The oocytes were divided into vitrified and non-vitrified groups. Vitrification was done b...

Abstract Study question Can increased time in thaw solution improve oocyte survival and outcomes following vitrification/warming? Summary answer Exposing vitrified oocytes to the first step thawing for 90 seconds improved compared 60 second exposure. What is known already Oocytes are a sensitive cell type careful handling of cells required obtain high vitrification warming. Carefully timed expo...

Abstract Compared to somatic or sperm cells, mammalian oocytes are much more sensitive cryopreservation mainly because of the large volume, cytoskeleton, and presence zona pellucida. Connections with surrounding cumulus cells also challenging preserve in immature oocytes. Besides genetic epigenetic information enclosed nucleus, contain organelles cytoplasmic factors that necessary for early emb...

BACKGROUND The mitochondria are an important source of adenosine triphosphate (ATP) production in pre-implantation embryo. Therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and ATP content. METHODS The embryos at 2-PN, 4-cell and blastocyst stages were collected from the oviduct of st...

This study was designed to compare the efficiency of the Cryotop and Calibrated plastic inoculation loop (CPIL) devices for vitrification of rabbit embryos on in vitro development and implantation rate, offspring rate at birth and embryonic and fetal losses. CPIL is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. In experiment 1, embryos we...

Ovulated mouse oocytes denuded of their cumulus cells, were vitrified in a solution containing 7 M ethylene glycol as the sole cryoprotectant using one or two steps of exposure before vitrification and were diluted in 1 M sucrose solution in 5 or 10 min after warming. The results proved that the viability of oocytes are detrimentally affected by exposure to the vitrification solution even witho...

introduction: the purpose of this study was to investigate the effect of vitrification procedure on the survival, fertilization, development and ultrastructure of metaphase (mii) mouse oocyte. materials and methods: female nmri mice 6-10 weeks old were superovulated using ip injection of 10 iu hmg and 10 iu hcg. ovulated oocyte were collected from the ampullary portion of the oviducts at 12-13 ...

The protocol of in vitro production (IVP) of bovine embryos is one of the critical factors determining embryo viability after cryopreservation. In this study were used two differents protocols to produce IVP bovine embryos, with variations in protein source, oocyte/zygote density per media volume, with the aim to determine the in vitro and in vivo embryo survival after vitrification using hand-...

BACKGROUND Obesity is associated with poor reproductive outcomes, but few reports have examined thawed embryo transfer in obese women. Many studies have shown that increased lipid accumulation aggravates vitrification injury in porcine and bovine embryos, but oocytes of these species have high lipid contents (63 ng and 161 ng, respectively). Almost nothing is known about lipids in human oocytes...