Technical and staining methods are described for making tangential sections using a specially prepared chuck surface in a cryostat. The method gives a rapid definitive diagnosis of Hirschsprung's disease in children.

Published experiments on natural turbulent convection in cryogenic (4)He gas show contradictory results in the values of Rayleigh number (Ra) higher than 10(11). This paper describes a new helium cryostat with a cylindrical cell designed for the study of the dependence of the Nusselt number (Nu) on the Rayleigh number (up to Ra approximately 10(15)) in order to help resolve the existing controv...

The LHC cryodipoles are composed of an evacuated cryostat and a cold mass, which is cooled by superfluid helium at 1.9 K. To obey constraints imposed by beam dynamics the particle beams must be centered within the mechanical axis of the dipole with a sub-millimeter accuracy. This requires in turn that the relative displacements between the cryostat and the cold mass must be monitored with accur...

2014 We describe a continuous-flow cryostat system with spherical geometry and full 360° freedom of motion for all three Euler angles. The cryostat does not increase the dead angular zones of the Euler-cradle itself. Any sample temperature between 3 K and 300 K can be set and kept gradient-free and constant by a computer controlled temperature regulation. The time constant of the device is in t...

A study was undertaken to determine if it might be possible to prepare tissue sections on slides without the use of paraffin embedding, microtome sectioning, or cryostat sectioning which involve equipment and training not always available to scholars or professionals wishing to examine tissue microscopically. After evaluating many different reagents, cutting instruments and solid supports, we d...

A new microassay in which cryostat sections of prostate tissue were used to provide the source of soluble androgen receptor for biochemical assay, was devised using an isoelectric focusing method, with [3H]-mibolerone as the androgenic radioligand. Adjacent cryostat sections from the same tissue block were stained for diagnostic and quantitative histological assessment. The assay was used to il...

We have developed a mini-coil pulsed magnet system with direct optical access, ideally suited for nonlinear and ultrafast spectroscopy studies of materials in high magnetic fields up to 30 T. The apparatus consists of a small coil in a liquid nitrogen cryostat coupled with a helium flow cryostat to provide sample temperatures down to below 10 K. Direct optical access to the sample is achieved w...

The top surface of boneless skinless chicken breasts was inoculated with either green fluorescent protein (GFP)-labeled Escherichia coli (E. coli-GFP) or rifampicin-resistant E. coli (E. coli-Rif) and subjected to electrically generated hydrodynamic shock wave treatment (HVADH). Cryostat sampling in concert with laser scanning confocal microscopy or plating onto antibiotic selective agar was us...