Ice cream is a kind of dairy products consumed all over the world and most popular among children and adults. However, ice cream available commercially is generally poor in natural antioxidants and vitamins. Thus, it is of interest to improve the nutritional values of ice cream using spiny bitter cucumber (Gac) which is a good source of lycopene and β-carotene along with probiotic bacteria. Fir...

Clinical trials are currently used to test therapeutic efficacies for lung cancer, infections and diseases. Animal models are also used as surrogates for human disease. Both approaches are expensive and time-consuming. The utility of human biospecimens as models is limited by specialized tissue processing methods that preserve subclasses of analytes (e.g. RNA, protein, morphology) at the expens...

Attempts to improve current methods of cryopreservation of corneas, whether by conventional freezing and thawing or by vitrification in the absence of ice, will require the use of high concentrations of cryoprotectants. In this study we extend our previous investigation of the tolerance of rabbit corneas to multimolar concentrations of the cryoprotectant dimethyl sulfoxide (Me2SO) added and rem...

Wood frogs (Rana sylvatica) exhibit marked geographic variation in freeze tolerance, with subarctic populations tolerating experimental freezing to temperatures at least 10-13 degrees Celsius below the lethal limits for conspecifics from more temperate locales. We determined how seasonal responses enhance the cryoprotectant system in these northern frogs, and also investigated their physiologic...

Experiments were designed to determine when, during the cryopreservation process, sperm lose fertilizing capacity and whether the cryoprotectant, methyl cellulose (MC), could be used in combination with glycerol to cryopreserve sperm and remain in the inseminate without reducing fertility. Semen diluted in Minnesota Avian extender (MNA) and inseminated immediately had greater fertility (75%) th...

Precision-cut tissue slices of both hepatic and extra-hepatic origin are extensively used as an in vitro model to predict in vivo drug metabolism and toxicity. Cryopreservation would greatly facilitate their use. In the present study, we aimed to improve (1) rapid freezing and warming (200 degrees C/min) using 18% Me(2)SO as cryoprotectant and (2) vitrification with high molarity mixtures of cr...

Mol. Nutr. Food Res. 2023, 67, 202200529 DOI: 10.1002/mnfr.202200221 Production conditions play a role in determining the probiotic properties of Streptococcus thermophilus. During manufacturing process S. thermophilus BT01, use an appropriate cryoprotectant led to significant reduction fecal urease activity healthy subjects during randomized controlled crossover study. This is reported by Anđe...

Long-term preservation of complex engineered tissues and organs at cryogenic temperatures in the absence of ice has been prevented to date by the difficulty of discovering combinations of cryoprotectants that are both sufficiently non-toxic and sufficiently stable to allow viability to be maintained and ice formation to be avoided during slow cooling to the glass transition temperature and subs...

Cryoprotection of horticultural products has been a matter of concern for the food industry in its search for efficient ways of improving the quality of frozen-thawed products. Research on the cryoprotection of plant tissues should make progress through a better understanding of the natural protective mechanisms of plant tissues during winter survival. The present study reports results on the u...