Squamous cell carcinoma of the head and neck (HNSCC) is the 6 leading cause of cancer by incidence worldwide with approximately 600,000 new cases per year. Unfortunately, only 40-50% of these patients will survive for 5 years. In order to study the immune response in patients with this disease we have developed a HNSCC tumor bank to compliment our Oral, Head and Neck Cancer Program. This tumor ...

BACKGROUND Germ cell depletion caused by chemical or physical toxicity, disease or genetic predisposition can occur at any age. Although semen cryopreservation is the first reflex for preserving male fertility, this cannot help out prepubertal boys. Yet, these boys do have spermatogonial stem cells (SSCs) that able to produce sperm at the start of puberty, which allows them to safeguard their f...

Recurrent Clostridium difficile infection (RCDI) is a severe and often fatal condition, killing an estimated 14,000 Americans per year. Treatment with faecal microbial transplantation (FMT) has been shown to be vastly superior to traditional antibiotic treatments, with success rates of around 90%. In studies performed so far, the stool (or its cryopreserved microbial content) of a healthy donor...

Abstract Study question This study investigated the take-up, hormonal profile and sperm quality in adolescents undergoing fertility preservation via different methods, prior to medical intervention for gender dysphoria. Summary answer Fertility surgical retrieval (SSR) or masturbation, is possible this group, however long-term data required check gamete health. What known already As increasing ...

Abstract Study question Does the transportation of vitrified embryos affect survival rate and in vitro fertilization (IVF) outcomes compared to those produced on-site? Summary answer The regulated transport cryopreserved has shown no detrimental effects on embryo or pregnancy outcomes. What is known already Reproductive medicine substantially evolved last decades. A growing number patients have...

Abstract Study question Which strategy is best for poor-prognosis patients: Two ICSI-PGTA cycles fresh oocytes (follicular plus luteal phase) or one cycle with vitrified and oocytes?" Summary answer Laboratory outcomes, ploidy rates clinical results after euploid blastocyst transfer are similar between the two DualStim/PGTA strategies. What known already Luteal phase ovarian stimulation (LPOS) ...

Abstract Study question Is there any relationship between the relative telomere length (RTL) within cumulus cells (CCs) and outcome of assisted reproductive treatment using corresponding oocyte? Summary answer Lower RTLs in CCs were significantly associated with embryos chosen for transfer or cryopreservation. In contrast, considered non-viable (discarded) tended to have higher RTLs. What is kn...

For a cryopreservation protocol to be successful or to provide optimal results, experimental conditions for each of its successive steps must be optimized. This paper presents some of the parameters, which have to be considered during the establishment of a cryopreservation protocol for clonal crop species. A prerequisite to the development of a cryopreservation protocol for any plant species i...

In the present study, we successfully cryopreserved entire blastoderms of rainbow trout, Oncorhynchus mykiss, embryos being at around midblastula stage of development. The method is a potential alternative to cryopreserving the whole fish embryo, not successful so far. key words. Blastoderm Blastomeres cryopreservation embryonic cells Trout. Cybium 2008, 32(2) suppl.: 139-141. (1) Faculty of Bi...

Deep in a freezer Japan, stash of tiny but exceptionally hardy micro-animals called tardigrades offered an even more surprising revelation. When thawed from their Rip Van Winkle–like slumber after 31 years, the seemed none worse for wear and began to reproduce. Inspired by natural processes that preserve features mummified tissue or permit full revival cells, tissues, entire organisms, scientis...