نتایج جستجو برای: cryoinjury

تعداد نتایج: 187  

2012
Yan Li Teng Ma

Human pluripotent stem cell (hPSC)-derived cell therapy requires production of therapeutic cells in large quantity, which starts from thawing the cryopreserved cells from a working cell bank or a master cell bank. An optimal cryopreservation and thaw process determines the efficiency of hPSC expansion and plays a significant role in the subsequent lineage-specific differentiation. However, cryo...

2015
Dorota Bednarek Juan Manuel González-Rosa Gabriela Guzmán-Martínez Óscar Gutiérrez-Gutiérrez Tania Aguado Carlota Sánchez-Ferrer Inês João Marques María Galardi-Castilla Irene de Diego Manuel José Gómez Alfonso Cortés Agustín Zapata Luis Jesús Jiménez-Borreguero Nadia Mercader Ignacio Flores

After myocardial infarction in humans, lost cardiomyocytes are replaced by an irreversible fibrotic scar. In contrast, zebrafish hearts efficiently regenerate after injury. Complete regeneration of the zebrafish heart is driven by the strong proliferation response of its cardiomyocytes to injury. Here we show that, after cardiac injury in zebrafish, telomerase becomes hyperactivated, and telome...

Journal: :Development 2011
Juan Manuel González-Rosa Víctor Martín Marina Peralta Miguel Torres Nadia Mercader

The zebrafish heart has the capacity to regenerate after ventricular resection. Although this regeneration model has proved useful for the elucidation of certain regeneration mechanisms, it is based on the removal of heart tissue rather than its damage. Here, we characterize the cellular response and regenerative capacity of the zebrafish heart after cryoinjury, an alternative procedure that mo...

Journal: :Journal of the Medical Association of Thailand = Chotmaihet thangphaet 2007
Thitikan Ngamwuttiwong Somboon Kunathikom

OBJECTIVE To evaluate cryodamage on human sperm motility, cryosurvival rate, and sperm chromatin assessed by acridine orange staining method (AO test) after a six-month freeze-thawing process using liquid nitrogen vapor. STUDY DESIGN Experimental study. MATERIAL AND METHOD Twenty normal semen samples were obtained from the male partner of infertile couples attending the infertility unit, Si...

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