A novel lateral flow immunoassay (LFIA) signal amplification strategy for the detection of Cry1Ab based on amplification via a polylysine (PL) chain and biotin-streptavidin system (BSAS) is described. In this system, multiple fluorescence dyes (FL) were directly coated on the surface of PL and conjugated with antibody via the BSAS for construction of novel signal amplification (FLPL-BSAS-mAb1) ...

Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa competed for those of Vip3Af.

Citation: Cheavegatti-Gianotto A, Gentile A, Oldemburgo DA, Merheb GdA, Sereno ML, Lirette RP, Ferreira THS and Oliveira WSd (2018) Lack of Detection of Bt Sugarcane Cry1Ab and NptII DNA and Proteins in Sugarcane Processing Products Including Raw Sugar. Front. Bioeng. Biotechnol. 6:24. doi: 10.3389/fbioe.2018.00024 lack of Detection of Bt sugarcane cry1ab and nptii Dna and Proteins in sugarcane...

Two strains of pink bollworm (Pectinophora gossypiella) selected in the laboratory for resistance to Bacillus thuringiensis toxin Cry1Ac had substantial cross-resistance to Cry1Aa and Cry1Ab but not to Cry1Bb, Cry1Ca, Cry1Da, Cry1Ea, Cry1Ja, Cry2Aa, Cry9Ca, H04, or H205. The narrow spectrum of resistance and the cross-resistance to activated toxin Cry1Ab suggest that reduced binding of toxin to...

In order to assess the risk that insecticidal transgenic plants may pose for bumblebees, we tested whether Bombus terrestris (L.) (Hymenoptera: Apidae) workers are able to detect insecticidal proteins dissolved in sucrose solution and whether consumption of these proteins will affect survival and offspring production. Feeders containing either Bacillus thuringiensis toxin (Cry1Ab), Kunitz soybe...

Field corn, Zea mays L., plants expressing Cry1Ab and Cry1F insecticidal crystal (Cry) proteins of Bacillus thuringiensis (Bt) Berliner are planted on considerable acreage across the Southern region of the United States. The fall armyworm, Spodoptera frugiperda (J.E. Smith), is an economically important pest during the mid-to-late season on non-Bt and some commercial Bt corn hybrids. The object...

Corn (Zea mays L.) transformed with a gene from the bacterium Bacillus thuringiensis (Bt) comprises 49% of all corn in the United States. The input of senesced corn tissue expressing the Bt gene may impact stream-inhabiting invertebrates that process plant debris, especially trichopteran species related to the target group of lepidopteran pests. Our goal was to assess risk associated with trans...

BACKGROUND The commercial release of rice genetically engineered to express a Cry1Ab protein from Bacillus thuringiensis (Bt) for control of Lepidoptera in China is a subject of debate. One major point of the debate has focused on the ecological safety of Bt rice on nontarget organisms, especially predators and parasitoids that help control populations of insect pests. METHODOLOGY/PRINCIPAL F...

A field population (SERD3) of Plutella xylostella L. collected in December 1994, and resistant to various insecticides, including Bacillus thuringiensis subsp. kurstaki (Btk) and B.t. subsp. aizawai (Bta), was selected with Btk (Btk-Sel) and Bta (Bta-Sel). Little evidence of cross-resistance was observed. Binding to midgut brush border membrane vesicles was examined for insecticidal crystal pro...

A shift toward transgenic crops which produce combinations of insecticidal proteins has increased the interest (Syngenta Seeds, Inc., Minnetonka, MN) in studying the potential for interactions amongst those proteins. We present a general testing method which accommodates proteins with nonoverlapping spectrums of activity. Our sequential testing approach first investigates groups of the proteins...