The crucian carp is an important aquaculture species and a potential model to study genome evolution and physiological adaptation. However, so far the genomics and transcriptomics data available for this species are still scarce. We performed de novo transcriptome sequencing of four cDNA libraries representing brain, muscle, liver and kidney tissues respectively, each with six specimens. The re...

Valnemulin (VML) is a semi-synthetic pleuromutilin derivative widely used to treat animal bacterial diseases. However, no study has comprehensively evaluated VML metabolism in aquatic animals, including crucian carp. This aimed investigate metabolites carp were quantified via intraperitoneal injection and analyzed ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Th...

Both adult females and males of Argulus mongolianus Tokioka, 1939 were collected from the buccal cavity wall body surface freshwater fishes in Lake Izunuma Uchinuma, Miyagi Prefecture, northen Honshu, Japan. The original description species was insufficient, based on a single female Inner Mongolia, China, no further has been made to date. Thus, this paper redescribes A. newly-collected specimen...

COMPARISON of microsporogenesis and microspore development A in a triploid Tradescantia has shown significant deviations from the normal diploid behavior. Occasional dicentric chromatids a t meiosis are found in diploid Tradescantias, but inversion crossovers in the triploid are much more frequent. An analysis of microspore development in a triploid Tradescantia shows that chromosome number has...

Hypoxia-inducible transcription factor-1 (HIF-1) is a master regulator of hypoxia-induced gene responses. To find out whether HIF-1 function is involved in gene expression changes associated with temperature acclimation as well as in hypoxia adaptation in poikilotherms, we studied HIF-1 DNA binding activity and HIF-1alpha expression in normoxia and during hypoxia (0.7 mg l(-1) O2) in crucian ca...

A new member of the inward-rectifier K(+) channel subfamily Kir2 was isolated and characterised from the crucian carp (Carassius carassius) heart. When expressed in COS-1 cells this 422 amino acid protein produced an inward-rectifying channel with distinct single-channel conductance, mean open time and open probability. Phylogenetic sequence comparisons indicate that it is not homologous to any...