One of the most extensively utilized rapid characterization, release and stability testing methods therapeutic proteins in biopharmaceutical field today is capillary SDS gel electrophoresis using borate cross-linked high molecular weight dextran. In spite its widespread use, however, composition dependent separation characteristics this very unique sieving matrix has not been investigated yet. ...

The colon is a promising site for drug targeting owing to its long transit time and mild proteolytic activity. The aim of this study was to prepare new low methoxy amidated pectin/NaCMC microspheres cross-linked by a mixture of Zn(2+) and Al(3+) ions and test their potential for colonic targeting of progesterone. A 2(4) factorial design was carried out to optimize the preparation conditions. Hi...

The present study aims at integrating drug-releasing materials with signal-processing biocomputing systems. Enzymes alanine transaminase (ALT) and aspartate transaminase (AST)—biomarkers for liver injury—were logically processed by a biocatalytic cascade realizing Boolean AND gate. Citrate produced in the system was used to trigger a drugmimicking release from alginate microspheres. In order to...

Stulak, John M., Luis A. Juncos, John A. Haas, and J. Carlos Romero. Systemic hemodynamics and renal function in hemorrhaged dogs resuscitated with cross-linked hemoglobin. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 278: R28–R33, 2000.—Cross-linked hemoglobin (XL-Hb) infused into dogs increases mean arterial pressure (MAP) but decreases blood flow to the renal (RBF), mesenteric (MBF)...

Preparation of chitosan (CS) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low CS molecular weight (MW) was investigated in this study. Diphtheria toxoid (DT) was used as a model antigen. The emulsion-solidification method was adopted for microencapsulation of DT.  In the first step, following the purification of semi-crude DT by the ion-exchan...