Methods In this cross sectional study, consenting 79 HIV+ve patients on antiretroviral therapy (for more than six months) visiting the Namakkal Government Hospital were recruited. Demography, anthropometry and ART regimens were collected. Patients’ self-perception of lipodystrophy was determined using standardized questionnaires and clinically confirmed. An overnight fasting blood was drawn to ...

Monoclonal antibodies were raised against cystozoites of Sarcocystis muris and characterised. Twelve monoclonal antibodies reacted in the ELISA, Dot-ELISA and IFAT only with homologous antigen. The other twelve showed cross reactions of various degrees with cystozoites of S. gigantea, S. tenella, S. arieticanis, S. capracanis, S. miescheriana and S. suihominis. Proteins of S. arieticanis, S. te...

We report three cases of invasive Geotrichum capitatum infection in patients with acute leukemia for which an enzyme-linked immunosorbent assay (ELISA) for Aspergillus galactomannan was positive, with no evidence of aspergillosis. Supernatants obtained from suspensions of 17 G. capitatum strains gave positive reactions with the Aspergillus galactomannan ELISA. These clinical and laboratory data...

A combination of ELISA, Western blotting and immunoaffinity concentration using an anti-ginsenoside Rb1 monoclonal antibody was applied for qualitative and quantitative surveys of ginsenoside Rb1 and related ginsenosides in roots and traditional Chinese herbal medicines. To improve the low correlation between ELISA and HPLC analysis, the crude extract of roots was immunoaffinity concentrated to...

Methods Cross-sectional retrospective study determining: 1) serum adalimumab (Ada) levels by a capture ELISA (positive > 5 ng/ml) and 2) levels of AAA by a two-site bridging ELISA [positive > 10 Arbitrary Units (AU)/ml]. The clinical activity was assessed by conventional tests and the physician visual analogue scale (ph-VAS) of 0 to 10, the same day that the blood sample was collected. Blood sa...

ELISA, cDNA dot blot hybridization and transmission by vector aphids were used to investigate the occurrence and degree of cross-protection produced in oat plants by virus isolates representing five strains or serotypes of barley yellow dwarf virus, namely PAV, MAV, SGV, RPV and RMV. Generally, the degree of cross-protection was positively correlated with the serological relatedness between the...

We report a case of cryptococcosis in which a serum enzyme-linked immunosorbent assay (ELISA) for Aspergillus galactomannan was positive, with no evidence of aspergillosis. Soluble antigens from 19 Cryptococcus neoformans strains and purified carbohydrates of C. neoformans capsule were thus assayed in the Aspergillus galactomannan ELISA. Antigens from all C. neoformans strains, and purified gal...

The aim of the present study was to develop a surface display ELISA (SD-ELISA) for IgG-serum reaction against bovine casein αS1 (CSN1S1). In a SD-ELISA, the antigen is displayed on the surface of Escherichia coli using the autodisplay technology and whole cells of E. coli are used to coat the microplates for serum testing. After establishing the setup of the SD-ELISA with polyclonal rabbit anti...

In this study, an enzyme-linked immunosorbent assay (ELISA) using glycoprotein and a monoclonal antibody (MAb) was developed for the detection of antibodies to vesicular stomatitis virus (VSV) serotype New Jersey (NJ). The glycoprotein to be used as a diagnostic antigen was extracted from partially purified VSV-NJ, and a neutralizing MAb specific to VSV-NJ was incorporated to compete with antib...