the aim of this study was to analyze the stress response during 3 hours net confinement stress and recovery period of 22 hours in normal (std) and in 17α-hydroxylase deficient common carp (e5). fish were raised for 6 months and sampled at t0 (control, unstressed), 5 minutes, 20 minutes, 1 hour and 3 hours of exposure to net confinement, and after 1 hour, 4 hours, and 22 hours of recovery. at ev...

Objective(s): In the present study the effect of stress on monkeys that had learned to retrieve food from a five-chamber receptacle, as well as the relationship between their behavior and the serum cortisol and epinephrine levels and relative size of the amygdala was evaluated. Materials and Methods: Six male rhesus monkeys were individually given access to the food reward orderly. They could e...

Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...

Apoptosis is a programmed cell death that has been attributed to the action of stress hormones, such as cortisol. We investigated the in vitro effect of cortisol in goldfish (Carassius auratus) mature (GSI: 2.75-4.65) or immature (GSI: 1.94-2.4) testes. Testes were taken out and transferred into culture medium (M 199), cut into 20 mm thick slices and supplemented with fetal bovine serum (FBS), ...

background: noise is one of the most important hazardous physical factors in industrial environments. this study aimed to determine the effects of noise exposure on serum cortisol level and some blood parameters among male workers of a steel production company. methods: this cross-sectional study was conducted on 50 male workers in a steel production company. in order to assess the changes in b...

cardiovascular adverse effects are interesting aspects of occupational noise exposure. one possible mechanism of these effects is an alternation in hypothalamic-pituitary-adrenal axis. our aim was to measure salivary cortisol response to relatively high-intensity noise exposure in a controlled randomized trial study. we exposed 50 male volunteers to 90 dba noise for 20 minutes and compared thei...

background: inadequate depth of anesthesia leads to release of stress hormones. electroencephalographic monitoring by bispectral index is a guide to asses the depth of anesthesia. the aim of the present study was to measure the serum cortisol levels as an index of stress response in patients who are candidates for coronary artery bypass graft surgery in two groups of patients.   methods: sevent...

to investigate the effect of exercise on cortisol responses and energy expenditure in obese and lean men, we measured the energy expenditure (ee) and salivary cortisol responses of six obese (age 22.83± 1.9 yr; vo2max,35.45±3.9 ml/min/kg; bmi 18.2±1.3 kg/m2) sedentary men. the protocol was running on a treadmill for 30 minutes with 65% maximal oxygen consumption. student t-test and one-way anov...

The unlabeled peroxidase-anti-peroxidase (PAP) method of Sternberger was used to localize cortisol within paraffin embedded sections of cat adrenal and pituitary tissue. Incubation of the cortisol antiserum used in this method with increasing concentrations of cortisol led to progressive extinction of cortisol staining of the adrenal fasciculata cells, (as measured with a scanning integrating m...