We have compared the covalent binding of two stereoisomeric benzo(a)pyrene-7,8-diol-9,10-epoxides to histones. From rat liver nuclei exposed to carcinogenic [3H]-(+/-)-7r,8t-dihydroxy-9t,10t-oxy-7,8,9,10-tetrahydrobenzo(a)p yrene [( 3H]BPDE-I) or noncarcinogenic [3H]-(+/-)-7r,8t-dihydroxy-9c,10c-oxy-7,8,9, 10-tetrahydrobenzo(a)pyrene [( 3H]BPDE-II), H1 and core histone fractions were prepared b...

RbAp48 (RbAp46 and RbAp48 are closely related proteins). Perhaps significantly, RbAp48 is associated with Department of Biology and Center for Molecular Genetics HDAC1 (Taunton et al., 1996) and is also a subunit of chromatin assembly factor 1 (for reviews, see Roth and A brief guide to the factors that were studied in each of the papers is given in Table 1. Chromatin structure is an important ...

The expression of core histone genes is cell cycle regulated. Large amounts of histones are required to restore duplicated chromatin during S phase when DNA replication occurs. Over-expression and excess accumulation of histones outside S phase are toxic to cells and therefore cells need to restrict histone expression to S phase. Misregulation of histone gene expression leads to defects in cell...

Eukaryotes tune the transcriptional activity of their genome by altering the nucleosome core particle through multiple chemical processes. In particular, replacement of the canonical H2A histone with the variants macroH2A and H2A.Z has been shown to affect DNA accessibility and nucleosome stability; however, the processes by which this occurs remain poorly understood. In this study, we elucidat...

in this study the effect of the alkylating agent, sulfur mustard, on calf thymus chromatin was investigated using uv/vis spectroscopy, gel electrophoresis and thermal denaturation techniques. the results show that treatment of isolated chromatin with sulfur mustard releases histones from the core particles but does not affect histone h i and nonhistone chromosomal proteins. the content of relea...

The Histone Database is a curated and searchable collection of full-length sequences and structures of histones and nonhistone proteins containing histone-like folds, compiled from major public databases. Several new histone fold-containing proteins have been identified, including the huntingtin-interacting protein HYPM. Additionally, based on the recent crystal structure of the Son of Sevenles...

BACKGROUND In eukaryotic cells, newly synthesized histone H4 is acetylated at lysines 5 and 12, a transient modification erased by deacetylases shortly after deposition of histones into chromosomes. Genetic studies in Saccharomyces cerevisiae revealed that acetylation of newly synthesized histones H3 and H4 is likely to be important for maintaining cell viability; the precise biochemical functi...

Gene activation requires alteration of chromatin structure to facilitate active transcription complex formation at a gene promoter. Nucleosome remodeling complexes and histone modifying complexes each play unique and interdependent roles in bringing about these changes. The role of distant enhancers in these structural alterations is not well understood. We studied nucleosome remodeling and cov...

Plasmodia of Physarum polycephalum can be induced to differentiate into dormant spherules: DNA-, RNA- and protein-synthesis cease during this process. Analysis of the histone H4 acetylation during spherulation revealed no significant changes of the relative acetate content and percentage of acetylated H4 subspecies. This result does not support a close correlation of histone acetylation and tra...