Unnoticed cell culture contamination by viruses, Mycoplasma, or other cell lines is not uncommon and a threat to laboratory safety and the quality of scientific results. We developed and validated a novel high-throughput Multiplex cell Contamination Test (McCT), which is currently able to detect 37 contamination markers in a single reaction. The assay is based on multiplex PCR with target-speci...

Abstract Background Predatory flagellates and ciliates are two common bio-contaminants which frequently cause biomass losses in Chlorella mass culture. Efficient targeted ways required to control these contaminations cultivation aiming for biofuel production especially. Results Five surfactants were tested its ability bio-contaminations All five able eliminate the contaminants at a proper conce...