The discovery that antigen}antibody interaction could be produced not only in liquids, but also on gel media, such as agar or agarose gels, with the formation of insoluble immunoprecipitates, opened the door to the development of the gel diffusion techniques for immunoprecipitation analysis. The Rrst of these techniques, known as double diffusion, was introduced by Ouchterlony in 1948. In this ...

Curli are extracellular amyloid fibres produced by Escherichia coli that are critical for biofilm formation and adhesion to biotic and abiotic surfaces. CsgA and CsgB are the major and minor curli subunits, respectively, while CsgE, CsgF and CsgG direct the extracellular localization and assembly of curli subunits into fibres. The secretion and stability of CsgA and CsgB are dependent on the ou...

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

expression of the growth hormone receptor (ghr) gene which is located on the sixteenth chromosome of sheep and its binding with gh is essential for growth and fat metabolism. in this study, blood samples were collected from the jugular vein from 88 sheep of kermani breed.dna was extracted from blood sample using the modified salting out method for amplification of 155 bp fragment containing a p...

Point mutations and small insertions or deletions in the human alpha-globin genes may produce alpha-chain structural variants and alpha-thalassemia. Mutations can be detected either by direct DNA sequencing or by screening methods, which select the mutated exon for sequencing. Although small (about 1 kb, 3 exons and 2 introns), the alpha-globin genes are duplicate (alpha2 and alpha1) and highly...

The most common technique for sorting, identifying, and purifying nucleic acid fragments is agarose gel electrophoresis. performance of the electrophoresis could be impacted by buffers’ composition, ionic strength, pH characteristics [1].

The pharmaceutical industry and most particularly the biopharmaceutical are demanding advancements in analytical techniques. field of proteomics peptide mapping needs sensitive analysis sophisticated instrumentation, which hyphenated techniques to be explored. In addition effective detection characterization, Due efficient selective separation, predominant focus has been drawn capillary electro...

We report that double-helical DNA constraints can be used to control the conformation of another molecule, RNA. When a covalently attached DNA constraint is structurally incompatible with the native Mg2+-dependent RNA conformation, RNA folding is disrupted, as revealed by nondenaturing gel electrophoresis and independently by chemical probing. Our approach is distinct from other efforts in DNA ...