Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

In this introduction to the forum Quirk Historicism, the editors describe the recent tendency of music scholars, in the wake of New Historicism, to avail themselves of objets trouvés and historical micronarratives for interpretation. Representations 132. Fall 2015 © The Regents of the University of California. ISSN 0734-6018, electronic ISSN 1533-855X, pages 61–78. All rights reserved. Direct r...

Twenty years after Poverty and Famines elaborated the entitlement approach as an innovative and holistic approach to famine analysis, debates about some of its fundamental assertions remain unresolved. This paper examines four limitations acknowledged by Sen himself: starvation by choice, disease-driven rather than starvation-driven mortality, ambiguities in entitlement speciŽ cation and extra-...

W. V. Quine famously argues that though all knowledge is empirical, mathematics is entrenched relative to physics and the special sciences. Further, entrenchment accounts for the necessity of mathematics relative to these other disciplines. Michael Friedman challenges Quine’s view by appealing to historicism, the thesis that the nature of science is illuminated by taking into account its histor...

Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the ...