Heterozygous replicative form molecules of bacteriophage phiX174 deoxyribonucleic acid (DNA) have been constructed in vitro. These are composed of viral strands extracted from purified preparations of phage bearing ts mutations and complementary strands of either half length or full length synthesized with purified DNA polymerase, in vitro, on DNA from am3 phage. In infections with such heteroz...

The fraction and distribution of DNA complementary to ribosomal RNA and preribosomal RNA was investigated by the technique of molecular hybridization with the DNA of isolated nucleoli and nuclei of rat liver. Nuclear DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.014% and 0.032%, respectively. Nucleolar DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.10% and 0.22%, respec...

We achieve very high hybridization efficiencies by using a new method to immobilize DNA strands on the surface of thermoresponsive polymer nanoparticles. Hybridization efficiencies of about 70 % are obtained between the DNA immobilized in the particles and a complementary strand in solution, even at very low ionic strengths (1 mM). The polymer nanoparticles have a glassy poly(methylmethacrylate...

A high mutation rate leading to tumor cell heterogeneity is a driver of malignancy in human cancers. Paradoxically, however, genomic instability can also render tumors vulnerable to therapeutic attack. Thus, targeting DNA repair may induce an intolerable level of DNA damage in tumor cells. BRCA2 mediates homologous recombination repair, and BRCA2 polymorphisms increase cancer risk. However, tum...

mature single-stranded DNA. The data clearly established that intact circles generated RNA complementary to only one strand, the complement of the mature strand in the duplex. Disrupted circles produced strands complementary to both DNA components. It appears that the strand selection mechanism which characterizes normal genetic transcription is a unique feature of circular double-stranded DNA....

Title of Document: TARGET ALKYLATION OF SINGLE AND DOUBLE STRAND DNA BY PEPTIDE NUCLEIC ACIDS Yang Liu, Doctor of Philosophy, 2011 Directed By: Professor Steven E. Rokita, Department of Chemistry and Biochemistry Quinone methides (QMs) generated in vivo can alkylate DNA and function as anti-cancer drugs. Delivery of QMs to target DNA is necessary to reduce the side effects caused by indiscrimin...

The synthesis of ribonucleic acid from ribonucleoside triphosphates by an enzyme system dependent on deoxyribonucleic acid has been described (2-7). The base composition of the RNA produced is identical with that of the DNA which is added. This result can be attributed, by an analogy with the complementary base model of DNA structure proposed by Watson and Crick (8), to hydrogen bonding by succ...

Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DN...

A novel fluorescent method for the detection of single nucleotide polymorphism (SNP) was developed using a hairpin DNA containing nucleotide base analog pyrrolo-deoxycytidine (P-dC) as a fluorescent probe. This fluorescent probe was designed by incorporating a fluorescent P-dC into a stem of the hairpin DNA, whose sequence of the loop moiety complemented the target single strand DNA (ss-DNA). I...

High-throughput immune repertoire sequencing is promising to lead to new statistical diagnostic tools for medicine and biology. Successful implementations of these methods require a correct characterization, analysis, and interpretation of these data sets. We present IGoR (Inference and Generation Of Repertoires)-a comprehensive tool that takes B or T cell receptor sequence reads and quantitati...