6 High sensitivity methods such as next generation sequencing and polymerase chain reaction (PCR) are adversely impacted by organismal and DNA contaminants. Current methods for detecting contaminants in microbial materials (genomic DNA and cultures) are not sensitive enough and require either a known or culturable contaminant. Therefore, high sensitivity methods not requiring a priori assumptio...

Microplastics (MPs) are prevalent in marine ecosystems. Because toxicants (termed here "co-contaminants") can sorb to MPs, there is potential for MPs to alter co-contaminant bioavailability. Our objective was to demonstrate sorption of two co-contaminants with different physicochemistries [phenanthrene (Phe), log10Kow=4.57; and 17α-ethinylestradiol (EE2), log10Kow=3.67] to MPs; and assess wheth...

With the aim of investigating the effects of carbonaceous sorbent amendment on plant health and end point contaminant bioavailability, plant experiments were set up to grow maize (Zea mays) in soil contaminated with polycyclic aromatic hydrocarbons (PAHs) and metals. Maize and pine derived biochars, as well as a commercial grade activated carbon, were used as amendments. Plant growth characteri...

The author used a recent article on lindane (γ-hexachloro-cyclohexane) reductive dechlorination by Fe/Pd bimetallics to point out that many other of published works in several journals do not conform to the state-of-the-art knowledge on the mechanism of aqueous contaminant removal by metallic iron (e.g. in Fe(0)/H(2)O systems). It is the author's view that the contribution of adsorbed Fe(II) to...

Multizone airflow network models assume that in a zone air temperature and contaminant concentrations are uniform, and air momentum effects are neglected. These assumptions could cause errors for airflow with strong buoyancy, large contaminant concentration gradient, or strong momentum. This study has found the correlations of the errors and some dimensionless air parameters. The assumption of ...

In this study, we tested a radical gas-based decontamination technique to prevent possible DNA contamination by the air and/or equipment used in molecular experiments. We prepared 10(4) molecules of model DNA contaminant and placed the dried DNA into test tubes, which were then exposed to radical gas. Quantitative PCR analysis showed that, even after a short exposure time of 30 minutes, 99.54% ...

This paper presents an analysis of the site restoration techniques that may be employed in a variety of contaminated site cleanup programs. It is recognized that no single specific technology may be considered as a panacea for all contaminated site problems. An easy-to-use summary of the analysis of the important parameters that will help in the selection and implementation of one or more appro...

BACKGROUND Despite the successful retrieval of genomes from past remains, the prospects for human palaeogenomics remain unclear because of the difficulty of distinguishing contaminant from endogenous DNA sequences. Previous sequence data generated on high-throughput sequencing platforms indicate that fragmentation of ancient DNA sequences is a characteristic trait primarily arising due to depur...

A system of impulsive ordinary differential equations is used to model the growth of microorganisms in a self-cycling fermentor. The microorganisms are being used to remove a non-reproducing contaminant that is limiting to growth at both high and low concentrations. Hence it is the concentration of the contaminant that triggers the emptying and refilling process. This model predicts that either...