Nine stable, auxotrophic, small-colony-forming variants of Staphylococcus aureus were isolated. They were of two types. One type, from broth cultures containing varying concentrations of gentamicin or kanamycin, required heme for normal growth. The second type, from the blood culture of a patient who had been treated with penicillin and oxacillin, required long-chain unsaturated fatty acids for...

An in vitro method for growing colonies of canine transplantable venereal tumor cells in a semisolid agar medium is described. Using autologous or pooled homologous normal dog sera as a feeder layer, 49.3 +/- 3.5 and 47.5 +/- 4.5 tumor colonies were obtained, respectively, when 2 X 10(4) tumor cells were plated. With this assay system, assessment of colony counts provided an accurate and rapid ...

Study of growth factor RNA levels in the stromal cells derived from the adherent layer of long-term bone marrow culture demonstrated constitutive expression of transforming growth factor beta 1 (TGF-beta 1) and macrophage colony-stimulating factor. These cells did not express granulocyte colony-stimulating factor, granulocyte-monocyte colony-stimulating factor, interleukin (IL) 1 alpha, IL-1 be...

OBJECTIVE To determine whether (1) a decreased concentration of Lactobacilli allows S. pyogenes to grow; (2) S. pyogenes is able to grow in the presence of healthy Lactobacillus concentrations; (3) S. pyogenes is capable of inhibiting Lactobacilli. METHODS One hundred fifty patient samples of S. pyogenes were mixed with four different concentrations of L. crispatus and L. jensenii. Colony cou...

Human urine contains growth factors; their physiological roles have not been established. The effect of normal human urine was examined in vitro on clonal growth of human bladder cancer cell lines. Clonal growth of HT-1376, HT-1197, and T24 was enhanced by five different fresh human urine samples from young men. Colony stimulating activity was detected in fractions with a molecular weight great...

The aim of this work was to assess OchratoxinA (OTA) production of different Aspergillus carbonarius isolates, evaluate their growth profile through different growth measurements, and reveal any underlying correlation between them. Ten different isolates of A. carbonarius isolated from Greek vineyards located in different geographical regions were examined in vitro for their OTA production pote...

Previous studies using unseparated normal human bone marrow cells have indicated that recombinant tumor necrosis factor a (rTNF-a) can inhibit the //; vitro colony growth by normal granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) progenitor cells in a dose-dependent manner. In the present studies, by using very low numbers of highly enriched normal bone marrow progenitor cell populations a...

The growth kinetics of Aspergillus nidulans, Mucor hiemalis and Penicillium chrysogenum on solid media and in submerged culture were studied. Growth of A. nidulans colonies on solid medium can be divided into the four phases of lag, exponential, deceleration and constant growth rate. The growth kinetics of A. nidulans in submerged culture were similar to those commonly found for unicellular mic...

America. CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. 2 Summary Siphonophores (Hydrozoa) have unparalleled colony-level complexity, precision of organization, and functional specialization between zooids (i.e., the units that make up colonies). Previous work has shown that, unlike o...