Matrilin-1 (cartilage matrix protein) is a homotrimeric protein that forms collagen-dependent and collagen-independent fibrils in the extracellular matrix of cartilage. In the growth plate of developing long bones, the gene for matrilin-1 is transcribed exclusively by the chondrocytes of the zone of maturation which is situated between the zones of proliferation and hypertrophy. When associated...

Collagen molecules are structural macro-molecules of the extracellular matrix that include in their structure one or several domains that have a characteristic triple helical conformation. They have been classified by types that define distinct sets of polypeptide chains that can form homo- and heterotrimeric assemblies. All the collagen molecules participate in supramolecular aggregates that a...

Precise mapping and unraveling the mechanism of interaction or degradation of a certain type of collagen triple helix requires the generation of short and stable collagenous fragments. This is a great challenge especially for hetero-trimeric collagens, where chain composition and register (stagger) are important factors. No system has been reported that can be efficiently used to generate a nat...

Previous studies show that the collagen fibrils of the mammalian vitreous humor are assembled largely from type I1 collagen with smaller amounts of type IX collagen and either type V or type XI collagen. In this paper, we report the separation of two chains of type V/type XI collagen from type I1 collagen by heparinSepharose chromatography. These chains were characterized by sequencing of selec...

hMSCs maintained in low-glucose and high-glucose DMEM for different cell passages. Low-glucose-maintained cells expressed up-regulated collagen types II and IX in both P6 and P9 cells. Fig 2. Gene expression of the TGF-β signaling molecules, TGF-β1, and TGF-β receptors 1 (TGFβR1) and 2 (TGFβR2) before and two hours after medium change. The expression levels were similar between lowand highgluco...