Competitive PCR (cPCR) is a quantitative PCR approach based on the addition of an internal standard to the PCR mixture. In this study, cPCR was used to quantitate genetically tagged cyanobacteria in Baltic Sea sediment. The cyanobacterium Synechocystis 6803-luc has a chromosomal insertion of the firefly luciferase gene, luc, as a marker detectable by PCR. A competitive standard was constructed ...

For definitional QA, we adopted a hybrid approach that combines several complementary technology components. Information retrieval (IR) was used to retrieve from the corpus the relevant documents for each question. Various linguistic and extraction tools were used to analyze the retrieved texts and to extract various types of kernel facts from which the answer to the question is generated. Thes...

the aim of this study was to determine the extent of metal pollutions and the identification of their major sources in the vicinity of the sangan iron mine occurring in ne iran. soil samples were collected from the vicinity of the mine site and analyzed for heavy metals. in addition, the chemical speciation of these metals was investigated by means of the sequential extraction procedure. the st...

Abstract: Classification of the cervical cell is one of the most important and crucial tasks in the medical image analysis. Due to its importance, the aim of the paper is to investigate about the classification of Cervical Cell as Normal Cell or Abnormal Cell by using individual feature extraction method and combining individual feature extraction features method with the classification techniq...

background: the aim of this study was to determine bioavailability heavy metal concentrations of cu, cd, cr, co and zn and health risk assessment in different types of klang district, selangor (malaysia) urban surface soil using in physiologically based extraction test in vitro human digestion model. methods: a total of 76 urban surface soil was sampled based on seven different types of land us...

A novel single phase co-solvent system using tetrahydrofuran (THF) promotes hydrolysis of maple wood to sugars, sugar dehydration, and lignin extraction simultaneously and achieves higher overall yields of the fuel precursors furfural, 5-hydroxymethylfurfural (HMF), and levulinic acid (LA) than previously reported from biomass. In a one-pot reaction, we obtained yields of 86% furfural, 21% HMF,...

A novel Schiff base-chitosan-grafted multiwalled carbon nanotubes (S-CS-MWCNTs) solid-phase extraction adsorbent was synthesized by covalently grafting a Schiff base-chitosan (S-CS) onto the surfaces of oxidized MWCNTs. The stability of a chemically(S-CS-MWCNTs) especially in concentrated hydrochloric acid which was then used as a recycling and pre-concentration reagent for further uses of (S-C...