The use of constitutive promoters to drive exogenous protein expression is an important tool for the study of diverse biological processes. To create and characterise a stably integrated expression system for DT40 cells, we constructed integration cassettes for three commonly used promoter elements; CMV (cytomegalovirus), CBA (chicken beta actin) or CAG (a hybrid promoter containing the CMV enh...

Cytomegalovirus (CMV) infection is usually inapparent in healthy adults but persists for life. Neural progenitor/stem cells are main CMV targets, and dentate gyrus (DG) a major neurogenic niche. Smaller DG volume has been repeatedly reported severe mental illness (SMI). Considering the suggested immune system, blood–brain barrier disturbances SMI, we hypothesized that exposure associated with s...

Gene transfer of a human cocaine hydrolase (hCocH) derived from butyrylcholinesterase (BChE) by 5 mutations (A199S/F227A/S287G/A328W/Y332G) has shown promise in animal studies for treatment of cocaine addiction. To predict the physiological fate and immunogenicity of this enzyme in humans, a comparable enzyme was created and tested in a conspecific host. Thus, similar mutations (A199S/S227A/S28...

Recombinant adeno-associated virus vectors (AAV) were prepared in high titer (10(12) to 10(13) particles/mL) for the expression of human factor IX after in vivo transduction of murine hepatocytes. Injection of AAV-CMV-F.IX (expression from the human cytomegalovirus IE enhancer/promoter) into the portal vein of adult mice resulted in no detectable human factor IX in plasma, but in mice injected ...

Cucumber mosaic virus (CMV) causes serious diseases in agricultural crops worldwide, predominantly vegetable and ornamental plants. A critical interaction has been reported between the plant Ferredoxin I (Fd I) protein with coat (CP) of M strain CMV (M-CMV) but not by CP Q (Q-CMV). To ascertain most likely symptom development model based on interacting Fd protein, further silico investigation w...

Background: Hematopoietic stem cell transplantation (HSCT) is an effective method for the treatment of hematological malignancies, severe aplastic anemia, and myelodysplastic syndromes. The most common infectious complication after HSCT cytomegalovirus (CMV) infection. purpose this study was to analyze status research related CMV infection by conducting a literature search CMV, hematopoietic, t...

Abstract Cytomegalovirus (CMV) is a virus that can be consider as invasive infection after transplantation or chemotherapy, long-term corticosteroid users or in immunodeficient patients such as HIV. Different complications were seen in immunocompetent patients but colitis rarely occurs. The diagnosis of CMV was based on pathology by colonoscopy, positive CMV antigen and high CMV-IgM titer serum...

BACKGROUND Measures to prevent transfusion-transmitted cytomegalovirus (TT-CMV) infection after hematopoietic stem cell transplantation (HSCT) include transfusion of CMV antibody-negative blood units and/or transfusion of leukoreduced cellular blood products. We assessed the incidence of TT-CMV in CMV-seronegative patients receiving CMV-seronegative HSC transplants, who were transfused with leu...