Materials and Methods: A total of 928 stool samples admitted to the laboratory during a four-month period were included in the study. Samples were diluted in saline and cultured in two EMB agar plates supplemented with either 1 μg/ml cefotaxime or 1 μg/ml ceftazidime. All isolates that grew were identified to the species level. E. coli and Klebsiella spp. strains were tested for ESBL production...

nosocomial infections of multidrug-resistant pseudomonas aeruginosa (mdrpa) are a growing concern in hospitalized patients in burn centers. the aim of this study was to investigate the flagellin profiling and antibiotic susceptibility of p. aeruginosa isolated from burn wound infections. during 8 month study, 73 clinically p. aeruginosa isolates collected from patients hospitalized in burn ward...

introduction: the major resistance mechanisms of pseudomonas aeruginosa to fluoroquinolones and carbapenems are associated with the mutations in the genes gyra and oprd encoding type ii topoisomerases (dna gyrase) and oprd porin, respectively. method: in this cross-sectional study, sixty five clinical samples were collected from patients hospitalized in al-zahra hospital of isfahan, iran. susce...

BACKGROUND The variety and number of laboratory quality standards, guidelines and regulations (hereafter: quality documents) makes it difficult to choose the most suitable one for establishing and maintaining a laboratory quality management system. OBJECTIVES There is a need to compare the characteristics, suitability and applicability of quality documents in view of the increasing efforts to...

Background & Aims: One of the main causes of nosocomial infection is pseudomonas aeruginosa and carbapenems is one of the most important classes of antibiotics used in the treatment of infections caused by this bacteria. Metallo-beta-lactamase (MBL) production is one of the most important mechanisms of resistance to carbapenems and their increased prevalence is a serious threat to treatment of ...

The Clinical and Laboratory Standards Institute recommends consideration of blaZ gene testing for cases of serious Staphylococcus aureus infection. Conventional PCR methods have demonstrated superior sensitivity and specificity to phenotypic tests. To our knowledge, this is the first description of real-time PCR detection of the blaZ gene.

Candida dubliniensis isolates tested for susceptibility to anidulafungin, caspofungin, and micafungin commonly showed artifactual regrowth and/or trailing effects with MIC tests done under conditions involving a high initial yeast concentration. The artifacts were less common with Candida albicans and seldom seen for either species under Clinical and Laboratory Standards Institute method M27-A ...

conclusions in our study, inducible clindamycin resistance rate was 4%; so it is necessary to conduct d-test regularly by disc diffusion for this bacterium. resistance to erythromycin, clindamycin, ciprofloxacin and gentamicin was significantly higher in mrsa isolates than methicillin susceptible staphylococcus aureus (mssa), although the methicillin resistance prevalence was low. background st...