Objective: To determine the effect of convallatoxin on K562 cell proliferation and apoptosis. Methods: CCK-8 assay was used to detect proliferation; PI staining, JC-1 Annexin V-FITC/PI double staining were analyze cycle, mitochondrial membrane potential, apoptosis; Western blotting cleaved caspase-9, caspase-3, Bcl-2, Bax, E2F1 expression Akt phosphorylation. Subsequently, AutoDock software int...

Ankylosing spondylitis (AS) is an autoimmune disease with unknown etiology. Dysregulated mesenchymal stem cells (MSCs) apoptosis may contribute to the pathogenesis of autoimmune diseases. However, apoptosis of MSCs from patients with AS (ASMSCs) has not been investigated yet. The present study aims to assess the apoptosis of bone marrow-derived ASMSCs and to investigate the underlying mechanism...

There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, a...

Caspase-3 is a cysteine protease that is most commonly associated with cell death. Recent studies have shown additional roles in mediating cell differentiation, cell proliferation and development of cell morphology. We investigated the role of caspase-3 in the development of chick auditory brainstem nuclei during embryogenesis. Immunofluorescence from embryonic days E6-13 revealed that the temp...

Calnexin is an endoplasmic reticulum (ER)-resident molecular chaperone that plays an essential role in the correct folding of membrane proteins. We found that calnexin is subjected to partial cleavage in apoptotic mouse cells. Both ER stress-inducing and ER stress-non-inducing apoptotic stimuli caused the cleavage of calnexin, indicating that this event does not always occur downstream of ER st...

IkB proteins function as direct regulators of Rel/ NF-kB transcription complexes. We show that the celldeath protease CPP32 (caspase-3) in vitro specifically cleaved chicken and human IkB-a at a conserved Asp-Ser sequence. This cleavage site appears to be identical to the site at which chicken IkB-a is cleaved in vivo in temperature-sensitive v-Rel-transformed chicken spleen cells undergoing ap...

The particulate matter 2.5 (PM2.5) from the chicken production system can cause lung injury and reduce productivity through prolonged breath as it attaches large amounts of harmful substances microbes. Melatonin has acted to regulate physiological metabolic disorders improve growth performance during poultry production. This research would investigate apoptosis caused by house PM2.5 on pulmonar...

objective: this study aimed to evaluate the incidence of apoptosis in vitrified and non-vitrified human ovarian tissue by the use of morphological analysis and apoptosis assay techniques. methods: we obtained human ovarian tissue biopsies from 30 women who underwent elective caesarean sections. tissues were transported to the laboratory in pre-warmed, equilibrated leibovitz l-15 medium within 2...

UVC irradiation induces oxidative stress and leads to cell death through an apoptotic pathway. This apoptosis is caused by activation of caspase-3 and formation of poly(ADP-ribose) polymerase-1 (PARP-1). In this study, the underlying mechanisms of Chlorella derived peptide (CDP) activity against UVC-induced cytotoxicity were investigated. Human skin fibroblasts were treated with CDP, vitamin C,...