dunaliella salina was isolated from maharlu salt lake in shiraz, iran, and was analyzed forthe effects of salinity stress on its growth, pigment content and ascorbate peroxidase activity. growth,expressed as number of cells ml-1, which was most rapid at 2 m nacl, was reduced at salinity extremes.during the exponential phase of growth, increasing salinity from 0.5 to 4 m nacl increased chl a fro...

We describe a quantitative paperchromatographic method for assaying 3-methoxy-4-hydroxymandelic acid (VMA) in urine. VMA is extracted from acidified urine into ethyl acetate, separated from interfering amine, acid, and alcoholic metabolites by paper chromatography, and reacted with diazotized p-nitroaniline. The colored derivative is eluted and spectrophotometrically analyzed. Recoveries of rep...

Quantify proteins without stable isotopes Label-free quantifi cation Quantitative data are derived from the number of MS/ MS spectra per peptide (spectral counting) or the integrated peak area of the ion chromatogram of a specifi c peptide. Generally considered less accurate than isotope-based methods but does not require expensive labeling reagents; method of choice for large numbers of sample...