in this study the effect of the alkylating agent, sulfur mustard, on calf thymus chromatin was investigated using uv/vis spectroscopy, gel electrophoresis and thermal denaturation techniques. the results show that treatment of isolated chromatin with sulfur mustard releases histones from the core particles but does not affect histone h i and nonhistone chromosomal proteins. the content of relea...

Fission yeast cut mutants cause cytokinesis in the absence of normal nuclear division. These mutants show abnormal uncoupled mitosis and are known to be the result of mutations in the genes encoding DNA topoisomerase II, proteins related to spindle pole duplication, and a kinesin-related mitotic motor. We have screened 717 temperature-sensitive (ts) mutants by individually observing their cytol...

skeletal abnormalities are most often used to describe defects in the arms or legs that are associated with genes or chromosomes, or that occur due to an event that happens during pregnancy. spider lamb syndrome (sls) is a congenital disorder in sheep breeding that is recognized by some deformities in skeletal system especially in the limbs.a dead day-old cross-breed white lamb with deformed li...

background: the particles in the range of 1-100 nm are called nanoparticles56t. gold nanoparticle is one of the most important metal nanoparticles with wide usage56t.objective: 56tthis study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice56t.materials and methods: 56tin this experimental study, 72 male bulb-c mice were divided into 9 groups inc...

introduction: to evaluate and compare efficiency of sil-select with percoll to recover sperm with normal chromatin in morphology and also studyeffect of these parameters on fertilization, embryo quality and cleavagescore. materials and methods: assessment of semen parameters, protamine deficiency (cma3) and excessive histone (aniline blue staining) were carried out before and after sperm proces...

In this study calf thymus chromatin was fractionated into active (S1 and S2) and inactive (P 2) chromatin. Then the interaction of an anthracyc1ine antibiotic, adriamycin, with these fractions was investigated employing absorption and difference UV Nis spectroscopy and SDS and agarose gel electrophoreses. The results suggest that the binding of adriamycin to the S 1 fraction is slight but ...

background: division of human genetics (dhg) is a referral center for karyotyping and counseling to the couples as well as to the individuals referred with bad obstetric history and infertility. materials and methods: from 1972 to 2003, overall 1666 couples and 131 female partners with bad obstetric history (boh) such as; spontaneous abortions, live births with congenital malformations and stil...

presence of extra roots and canals should be considered before initiation of root canal treatment for the success of endodontic treatment. a mandibular second premolar with three separate roots is very rare and its prevalence has been reported to be around 0.1%. this case report explains non-surgical endodontic treatment of a mandibular second premolar with three separate roots and three separa...

gibberellic acid (ga3) is a group of plant hormones identified in various plants. the aim of this study was to determine the effects of ga3 on sperm parameters and in vitro fertilization (ivf). fifty six adult male rats were divided into seven groups as, control, treatment and sham. following 15, 30 and 45 days of ga3 and methanol alcohol (ma) administration, rats were euthanized and epididymis...

OBJECTIVE To evaluate levels of sperm nuclear DNA damage in infertile men with normal and abnormal standard semen parameters. DESIGN Prospective study. SETTING Male infertility clinic. PATIENT(S) Ninety-two men seeking infertility treatment and 16 fertile volunteers. INTERVENTION(S) Standard semen analysis was performed according to the World Health Organization guidelines. MAIN OUTCO...