نتایج جستجو برای: cho cell mutant

تعداد نتایج: 1770578  

Journal: :Moscow University Biological Sciences Bulletin 2021

For the first time, a patient with asymptomatic QT interval prolongation was found to carry missense mutation c.277G>A (p.Val93Ile) in KCNJ2 gene, described previously only as cause of familial form atrial fibrillation. The corresponding amino acid substitution introduced into plasmid encoding Kir2.1 channel and mutant gene expressed Chinese hamster ovary cells (CHO-K1) evaluate effect on IK1 c...

Journal: :Journal of virology 1977
R A Lamb P W Choppin

Influenza virus polypeptides were not synthesized in wild-type CHO-S-infected cells in the presence of alpha-amanitin, but were synthesized in CHO-Amal cells, a mutant cell line whose DNA-dependent RNA polymerase II is specifically resistant to this drug, indicating that this cellular enzyme is involved in influenza virus replication. The results of experiments designed to detect viral polypept...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1995
K Detjen J Yang C D Logsdon

Cellular desensitization is believed to be important for growth control but direct evidence is lacking. In the current study we compared effects of wild-type and down-regulation-resistant mutant m3 muscarinic receptors on Chinese hamster ovary (CHO-K1) cell desensitization, proliferation, and transformation. We found that down-regulation of m3 muscarinic acetylcholine receptors was the principa...

Journal: :Molecular biology of the cell 2001
S Yamashiro H Chern Y Yamakita F Matsumura

Caldesmon is phosphorylated by cdc2 kinase during mitosis, resulting in the dissociation of caldesmon from microfilaments. To understand the physiological significance of phosphorylation, we generated a caldesmon mutant replacing all seven cdc2 phosphorylation sites with Ala, and examined effects of expression of the caldesmon mutant on M-phase progression. We found that microinjection of mutan...

Journal: :Nucleic acids research 1991
M. Heffernan J. W. Dennis

Eukaryotic expression vectors have been used successfully in viral LT-expressing cell lines (ie. COS) to clone cDNAs encoding proteins that can be detected through their bio-activity or reactivity with specific antibodies. Since Chinese hamster ovary cells (CHO) have been used extensively for the isolation and characterization of somatic cell mutants, we felt it would be an advantage to develop...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1989
R König G Ashwell J A Hanover

CD4 is a T-cell surface glycoprotein and serves as the receptor for the human immunodeficiency virus. Glycosylation of CD4 has been shown to be necessary for proper surface expression. To study the biosynthesis and assembly of CD4, wild-type and glycosylation-deficient mutant Chinese hamster ovary (CHO) cells were cotransfected with a cDNA encoding CD4 and a cDNA for the human multiple drug res...

Journal: :Infection and immunity 2000
M S Chaussee R L Cole J P van Putten

Streptococcus pyogenes secretes several proteins that influence host-pathogen interactions. A tissue-culture model was used to study the influence of the secreted cysteine protease streptococcal erythrogenic toxin B (SPE B) on the interaction between S. pyogenes strain NZ131 (serotype M49) and mammalian cells. Inactivation of the speB gene enhanced fibronectin-dependent uptake of the pathogen b...

2007
Shunichi Yokoe Motoko Takahashi Michio Asahi Seung Ho Lee Wei Li Daisuke Osumi Eiji Miyoshi Naoyuki Taniguchi

ErbB2 and ErbB3, two members of the ErbB family, form a high-affinity heregulin coreceptor that elicits potent mitogenic and transforming signals, and clinical studies indicate that these receptors play an important role in tumor incidence and progression. To determine whether N-glycosylation is involved in the function of ErbB3, a series of human ErbB3 molecules devoid of N-glycans were prepar...

Journal: :Cancer research 2007
Shunichi Yokoe Motoko Takahashi Michio Asahi Seung Ho Lee Wei Li Daisuke Osumi Eiji Miyoshi Naoyuki Taniguchi

ErbB2 and ErbB3, two members of the ErbB family, form a high-affinity heregulin coreceptor that elicits potent mitogenic and transforming signals, and clinical studies indicate that these receptors play an important role in tumor incidence and progression. To determine whether N-glycosylation is involved in the function of ErbB3, a series of human ErbB3 molecules devoid of N-glycans were prepar...

Journal: :The Journal of biological chemistry 1999
V M Gordon K L Nelson J T Buckley V L Stevens R K Tweten P C Elwood S H Leppla

The alpha toxin produced by Clostridium septicum is a channel-forming protein that is an important contributor to the virulence of the organism. Chinese hamster ovary (CHO) cells are sensitive to low concentrations of the toxin, indicating that they contain toxin receptors. Using retroviral mutagenesis, a mutant CHO line (BAG15) was generated that is resistant to alpha toxin. FACS analysis show...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید