This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The procedure enables direct translation of any published equation developed on a 1-cm pathlength spectrophotometer to any format microplate with any sample volume. Chlorophylls were extracted with methanol because it is compatible with cheap polystyrene plates; however, the principles apply t...

We compare the chlorophyll fluorescence decay kinetics of the wild type and the D2-H117N mutant photosystem II reaction centers isolated from Chlamydomonas reinhardtii. The histidine residue located at site 117 on the D2 polypeptide of photosystem II is a proposed binding site for one of two peripheral accessory chlorophylls located in the reaction center complex. The peripheral accessory chlor...

UV-B radiation (280-315 nm) incident on the leafy liverwort Cephaloziella varians growing at Rothera Point on the Western Antarctic Peninsula was manipulated in late austral spring 1998 by screens consisting of a novel combination of plexiglass panes and polyester sheets. The screens transmitted c. 79%, 68%, 48%, 41% and 31% of daily UV-B radiation dose. Plants from under and outside the screen...

This article reflects the results of studies pigment system 15 species woody plants in Altai Botanical Garden. The data obtained indicate presence specificity content chlorophylls a, b and carotenoids. In studied species, chlorophyll a varies from 5.38 mg/l to 19.28 mg/l, 0.43 5.09 carotenoids 4.65 8.34 .

Chlorophyll degradation is an aspect of leaf senescence, which is an active process to salvage nutrients from old tissues. non-yellow coloring1 (nyc1) is a rice (Oryza sativa) stay-green mutant in which chlorophyll degradation during senescence is impaired. Pigment analysis revealed that degradation of not only chlorophylls but also light-harvesting complex II (LHCII)-bound carotenoids was repr...

Nanochlorum eucaryotum is a very small species of unicellular coccoid green algae (1.5 nm). The growth of Nanochlorum under different conditions of salinity, pH and light intensities was studied. Optimal growth rates were observed with normal sea water salinity and low light conditions at pH 7.0. The contents of chlorophylls, carotinoids, soluble proteins and the chlorophyll a to chlorophyll b ...

Russian wheat aphid, Diuraphis noxia (Mordvilko), feeding injury on 'Betta' wheat isolines with the Dn1 and Dn2 genes was compared by assessing chlorophyll and carotenoid concentrations, and aphid fecundity. The resistant Betta isolines (i.e., Betta-Dn1 and Betta-Dn2) supported similar numbers of aphids, but had significantly fewer than the susceptible Betta wheat, indicating these lines are re...

Siegrid Schoch3 and M onica Ihl a Botanisches Institut, Universität München, D-80638 München, Menzingerstr. 67, Germany b Departamento de Ingeniena Quimica, Universidad de La Frontera, cas. 54-D, Temuco, Chile Z. Naturforsch. 53c, 21-26 (1998); received September 19/October 11, 1997 Chlorophyll, Chlorophyllase, Substrates Specificity, Vegetables, Zn pheophytin The activity of chlorophyllase (ch...

Senescence is a highly organized and well-regulated process. As much as 75% of total cellular nitrogen may be located in mesophyll chloroplasts of C(3)-plants. Proteolysis of chloroplast proteins begins in an early phase of senescence and the liberated amino acids can be exported to growing parts of the plant (e.g. maturing fruits). Rubisco and other stromal enzymes can be degraded in isolated ...