Rat liver cells in vitro were transformed with chicken sarcoma virus B77, giving RL(B77) cells, and with murine sarcoma virus (Harvey), giving RL(MSV) cells. Rat liver cells transformed spontaneously in vitro were designated RL cells. In addition, the RL(MSV) cell line was adapted for growth in culture fluid containing 25 mug of 5-bromodeoxyuridine per ml. All cell lines were tumorigenic in 1-w...

The growth kinetics of avian infectious bronchitis (AIB) virus in vitro have been investigated previously using different systems, including embryonated chicken eggs (Hitchner and White, 1955), and cell cultures of chick embryo kidney (Lukert, 1965 ; Bulow, 1966; Akers and Cunningham, 1968) or chick embryo liver (Lukert, 1965). Organ cultures of ciliated epithelium have been employed in various...

All viruses infecting fish must cross the surface mucosal barrier to successfully enter a host. Infectious salmon anaemia virus (ISAV), the causative agent of the economically important infectious salmon anaemia (ISA) in Atlantic salmon, Salmo salar L., has been shown to use the gills as its entry point. However, other entry ports have not been investigated despite the expression of virus recep...