1. Antisera have been produced in chickens with specific precipitates from Type II pneumococcus horse and rabbit antisera. 2. Specific precipitates from anti-Types I and II pneumococcus horse sera removed the same amount of antibody from the chicken anti-horse specific precipitate serum. Specific precipitates from horse antisera to diphtheria toxin and to crystalline egg albumin removed about o...

Heterokaryons derived from polyethylene glycol-mediated fusion of myoblasts at different stages of development were used to investigate the transition of cells in the skeletal muscle lineage from the determined to the differentiated state. Heterokaryons were analyzed by immunofluorescence, using rabbit antibodies against the skeletal muscle isoforms of chicken creatine kinase and myosin, and a ...

background: toxoplasma gondii, as an important food-borne protozoan parasite, occurs widely in humans and animals including domestic poultry throughout the world. the goal of this survey was to determine prevalence rate of t. gondii in free-ranging chickens sold in tunis, tunisia. methods: in the present study, the prevalence of t. gondii in 40 free-range chickens (gallus domesticus) marketed i...

A common approach for developing diagnostic tests for influenza virus detection is the use of mouse or rabbit monoclonal and/or polyclonal antibodies against a target antigen of the virus. However, comparative mapping of the target antigen using antibodies from different animal sources has not been evaluated before. This is important because identification of antigenic determinants of the targe...

Rabbit polyclonal antibodies directed against the chicken neural cell adhesion molecule (N-CAM) were used to isolate four overlapping cDNA clones from a chicken cDNA expression library in bacteriophage gamma gt11. These clones collectively accounted for 3.8 kilobases of N-CAM mRNA sequence and hybridized specifically to two 6-7-kilobase brain polyadenylated RNA species that co-migrated with pre...

Protein arrays are frequently used to profile antibody repertoires in humans and animals. High-throughput protein array characterisation of complex antibody repertoires necessitates the use of extensively validated secondary detection antibodies. This article details the validation of an affinity-isolated anti-chicken IgY antibody produced in rabbit and a goat anti-rabbit IgG antibody conjugate...

Newcastle disease (ND) causes severe economic losses in poultry production. Despite the intensive vaccination regimes of NDV in Egypt, many outbreaks are being reported. The present study focused on the preparation and evaluation of inactivated velogenic Newcastle disease virus vaccine (genotype VII) isolated from Egyptian broiler chicken during 2015-2016. Fifty-five tissue samples including tr...