Stimulator of IFN genes (STING) is an adaptor that functions downstream of retinoic acid-inducible gene I (RIG-I) in mammalian cells; however, RIG-I is absent in chickens. We identified chicken STING (chSTING) as a critical mediator of virus-triggered type I IFN signaling in RIG-I-null chicken cells. Overexpression of chSTING in DF-1 cells inhibited Newcastle disease virus and avian influenza v...

Chicken cells of chicken helper factor-positive (chf+) phenotype were infected with a cloned (envE-free) Rous sarcoma virus, subgroup D, and examined for the presence of parent and recombinant proviruses by transfection in chicken and turkey cells, respectively. It was found that most parent virus DNA is integrated into the host cell genome during the first 18 hr after infection, and no signifi...

Chicken embryonic eggs and Madin-Darby canine kidney (MDCK) cell line were used for comparing the propagation of avian influenza A (H5N1) virus (C2105Dx1, a Thai isolate). The growth of the H5N1 virus was determined by using HA test and 50% infectivity dose to assess the suitability of the systems supporting the propagation of the virus. The results indicated that the Thai H5N1 virus was propag...

Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide and the pathogenicity of Newcastle disease virus (NDV) strains varies with different virulence. Samples were collected from chicken commercial farms in Iran during 2014–2015. ND virus were characterized (NDV) by partial sequences...

Newcastle disease (ND) is one of the most serious illnesses of chickens. Live vaccines are widely used to prevent chicken from the disease all over the world. To access the effective and potentiate ND vaccine, a homogenous subpopulation from LaSota strain was selected following cultivation of the virus on primary chicken embryofibroblast (CEF) cells. Pathogenicity indices and molecular characte...

Background and Aims: Finding prevalence of chicken anemia virus (CAV) infection in native chickens is necessary to avoid transmission of infection to commercial flocks. In this paper we attempt to describe molecular detection of chicken anemia virus in native Larry-breed chickens in Chaharmahal-va-Bakhtiyari province in Iran for the first time. Materials and Methods: Blood samples were collect...

RNA sequence relatedness among avian RNA tumor virus genomes was analyzed by inhibition of DNA-RNA hybrid formation between 3H-labeled 35S viral RNA and an excess of leukemic or normal chicken cell DNA with increasing concentrations of unlabeled 35S viral RNA. The avian viruses tested were Rous associated virus (RAV)-3, avian myeloblastosis virus (AMV), RAV-60, RAV-61, and B-77 sarcoma virus. H...