The method using lucigenin-dependent phagocytic chemiluminescence is described for the assessment of alveolar macrophage metabolic activity in response to stimulation by opsonised particles or soluble agents. The requirement for superoxide anion (O2-) in the production of chemiluminescence is suggested by inhibition (95%) using superoxide dismutase. The results obtained are correlated with thos...

PURPOSE To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of ...

A novel flow-injection chemiluminescence(CL)method has been developed for the determination of hydroquinone (HQ) and catechol (CT), based on their inhibition of the chemiluminescence reaction of luminol–KMnO4–β-cyclodextrin (β-CD). It was found that β-cyclodextrin could effectively enhance the chemiluminescence produced from the reaction of luminol with KMnO4 in basic media. The proposed method...

Chemiluminescence, Dioxetanes, Reaction Kinetics, Spectra, Biradicals The thermal decomposition of 1,2-diadamantyldioxetane was studied by kinetic and spectroscopic methods. Spectra of the chemiluminescence emitted during the thermally induced decomposition of 1,2-diadamantyldioxetane, tetramethyldioxetane and trimethyldioxetane were obtained and the influence of quenchers and radical-scavenger...

The chemiluminescence of the luminol-H2O2-horseradish peroxidase system is increased by fluorescein. Fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. The maximum intensity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than th...