PURPOSE The objective of this project was to establish an 'in-house' DNA database to store and compare profiles genotyped in the Department of Forensic Medicine, Medical University of Bialystok. MATERIAL AND METHODS DNA was extracted using Chelex-100, an organic procedure, or commercial kits. Genetic profiles were obtained using AmpFISTR SGM Plus, AmpFISTR Profiler, or AmpFISTR Identifiler an...

Cytosols contain a heat-stable, chelatable, anionic, molybdate-like factor that stabilizes glucocorticoid receptors in a heteromeric complex with hsp90 (refers to the 90-kDa heat shock protein) and inhibits their transformation to the DNA-binding state (Meshinchi, S., Grippo, J.F., Sanchez, E.R., Bresnick, E.H., and Pratt, W.B. (1988) J. Biol. Chem. 263, 16809-16817). In this work, we demonstra...

Trace mineral studies involving metal ion chelators have been conducted in investigating the response of gene and protein expressions of certain cell lines but a few had really focused on how these metal ion chelators could affect the availability of important trace minerals such as Zn, Mn, Fe and Cu. The aim of the present study was to investigate the availability of Zn for the treatment of MC...

BACKGROUND Neonatal screening for metabolic diseases, involving samples stored on filter paper (Guthrie spots), provides a potential resource for genetic epidemiological studies. OBJECTIVE To develop a method to make these dried blood spots available for large scale genetic epidemiology. METHODS DNA from untraceable Guthrie spots was extracted using a saponin and chelex-100 based method and...

Freshwaters are recognized as dynamic systems that may be far-removed from equilibrium. A kinetic approach using the competing ligand exchange method with Chelex 100 as the competing ligand and inductively coupled plasmamass spectrometry to measure the dissociation kinetics was used to investigate the chemical speciation of Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), and Pb(II) in model sol...

with impervious exteriors, such as shells or chitinous exoskeletons. Advantages to this method are high throughput, high success rate and enough template DNA for several different assays (FRE-PCR used only 5 μL of the 20-μL supernatant). Other extraction methods that were tried and discarded for low success rates included boiling in water or PCR buffer, grinding before boiling, grinding with co...

OBJECTIVE The aim of this study was to compare and evaluate three methods of DNA extraction for the amplification of Chlamydia trachomatis in uterine cervical samples collected in PreservCyt solution. ThinPrep is the trade name for the slide preparation. METHODS Thirty-eight samples collected in LCx buffer medium, which were identified as C. trachomatis infected by ligase chain reaction (LCR)...

Hyperpolymorphic short tandem repetitive DNA sequences, STRs or microsatellites, have become widely used in human identification, particularly in criminal cases and in mass disasters. In such cases the substrates for the analyses may be decomposed biological material, a fact that has to be taken into account when choosing the appropriate casework methods. In this paper we report the evaluation ...

Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico, 20122 Milan, Italy Blood samples collected with heparin as anticoagulant have been shown to yield decreased quantities of DNA, and an inhibitory effect of this anticoagulant on PCR has been demonstrated. Furthermore, in our experience, the amplification by PCR of HLA-DRB1 second exon was unsuccessful when the DN...