An enzyme-linked immunosorbent assay (ELISA) for Lawsonia intracellularis was developed and compared with a whole-cell antigen-based immunofluorescence antibody test (IFAT). The antigen-containing lipopolysaccharide (LPS) was derived from Percoll gradient purified cultures of L. intracellularis by using a modification of the Westphal hot phenol procedure. The antigen was bound directly to polys...

Nonsteroidal and steroidal drugs are generally used to treat inflammation. However, these drugs have side-effects like nausea, vomiting, etc. This lead to search for new anti-inflammatory agents from natural sources, which would be effective and safe. Many plant extracts show a synergistic effect with each other or with modern drugs. The aim of the present study is to evaluate the synergistic a...

Lawsonia inermis L. (= L. alba Lamk.), belonging to the Lythraceae family, is a very widespread medicinal plant and natural dye in the world. This herb which has interesting dyeing properties was used traditionally for centuries in Asia, in Africa for dyeing hair and painting skin and nails. Since 1890, it has been widely distributed to Europe and nowadays available in worldwide market as ingre...

Lawsonia inermis Linn (Lythraceae), also known as henna, is a small shrub or tree distributed throughout Taiwan's Lanyu Island, in North Africa, and in Australia. Its leaves are used as a folk medicine for the treatment of external hemorrhage and fingernail abscesses. Investigation of the ethyl acetate (EtOAc)-soluble fractions from methanol extract of the aerial part of Lawsonia inermis has le...

The antioxidant activity of several Malaysian plant extracts was analyzed simultaneously with their pro-oxidant capacity. This ratio represents an index (ProAntidex) of the net free radical scavenging ability of whole plant extracts. We observed that ethanolic extracts of Nephelium lappaceum peel, Fragaria x ananassa leaf, Lawsonia inermis leaf, Syzygium aqueum leaf and grape seed had a lower P...

To characterize the immune response associated with Lawsonia intracellularis infection, twenty-eight, 7-week-old pigs were dosed orally with a pure culture of L. intracellularis. Animals were killed 3, 7, 14, 21, 28, 35, and 42 days postinfection. Light microscopic studies were undertaken to immunophenotype the immunologic response using specific antibodies to T-cell subsets (CD3, CD4, and CD8)...