Cellulose is the most plentiful renewable biopolymer in nature which could be utilized by cellulolytic enzymes. Cellulases are among the most important groups of industrial enzymes which are widely consumed in biofuel production, pulp and paper, textile, and detergent industries. These enzymes can support a cleaner environment through reducing chemical processes in mentioned industries and agro...

Background A recently constructed cellulolytic Yarrowia lipolytica is able to grow efficiently on an industrial organosolv cellulose pulp, but shows limited ability to degrade crystalline cellulose. In this work, we have further engineered this strain, adding accessory proteins xylanase II (XYNII), lytic polysaccharide monooxygenase (LPMO), and swollenin (SWO) from Trichoderma reesei in order t...

Coriolopsis rigida was isolated as an endophytic fungus from the medicinal plant Cochlospermum regium. The cultivated on solid media containing rice or pineapple crown bran for analyzing production of extracellular enzymes. activities lipase, β-xylosidase, pectinase, fructosyltransferase (FTase), invertase, α-amylase and total cellulolytic (FPase) were determined. showed positive activity all e...

Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently limited to a few soil-isolates. Here, we demonstrate the biomass-deconstructing capability of St...

Studies in this and other laboratories have disclosed a variety of starch-digesting rumen organisms, including Streptococcu bouis (Hungate et al., 1952), several cellulolytic strains (Hungate, 1950), and various non-cellulolytic bacteria (Gall et al., 1947; Gall and Huhtanen, 1951; Huhtanen and Gall, 1953a, b; Bryant and Burkey, 1953a,b; Jayko, 1953; McPherson, 1953; van der Wath, 1948) as well...

Reliable, rapid and inexpensive detection of cellulolytic enzymes that can be used for a wide variety of biological and environmental samples are currently in high demand. Here, a new cellulase detection protocol is described that circumvents problems observed with popular agar-based methods by exploiting the ability of carboxymethylcellulose (CMC) to form gel-like surfaces on its own. These pu...

To understand the ability of producing cellulolytic enzyme activity in the sapstaining fungi, four species of Ophiostoma and two species of Leptographium were investigated in the culture media containing each of cellulose substrates such as CM-cellulose, Avicel and D-cellobiose and each of chromogenic dyes such as Congo-Red, Phenol Red, Remazol Brilliant Blue and Tryphan Blue. When the fungi we...

Ragi Tapai is a starter used for the fermentation of cassava and glutinous rice to produce conventional fermented products, such as in Indonesia. contains several enzymes microbes that help process. The purpose this study was identify most potential amylolytic yeast in-vitro (amylolytic, cellulolytic, fermentative). GPA, YEA, APB, CMCA, GPACaCO3 were medium selection characterization. result sh...

Bacteria and fungi are thought to degrade cellulose through the activity of either a complexed or a noncomplexed cellulolytic system composed of endoglucanases and cellobiohydrolases. The marine bacterium Saccharophagus degradans 2-40 produces a multicomponent cellulolytic system that is unusual in its abundance of GH5-containing endoglucanases. Secreted enzymes of this bacterium release high l...