Statement of Purpose: Complex structural organization in the human body is a key factor to produce the appropriate tissue functionality. In mature skeletal muscle, the muscle fibers are highly oriented and the bundle structure is essential to produce its mechanical functions. Therefore, a technique for organizing the microstructure of native muscle tissue is required to produce a biomimetic eng...

A light-addressable potentiometric sensor (LAPS) is a semiconductor-based chemical sensor, in which the measurement area defined by probe light illuminating plate. In this study, novel method to detect hydrogen permeation through metal was proposed using system, LAPS combined with micro-volume cell. The sensing surface of placed vicinity exit an iron sheet form cell filled Na2HPO4 solution, and...

We have developed a new cell transplantation technique, in which mesenchymal stem cells (MSCs) were cultured and lifted as a cell sheet structure. The sheet formed bone tissue after in vivo transplantation at a subcutaneous site, without the need for any scaffold. The sheet could also be transplanted with a scaffold, such as hydroxyapatite (HA). Transplantation of this sheet/HA construct result...

Tissue engineering requires novel technologies for establishing 3D constructs, and the layered method of culturing cell sheets (cell sheet engineering) is one potentially useful approach. In the present study, we investigated whether coating the culture surface with RGD (Arg-Gly-Asp) peptide-conjugated magnetite cationic liposomes (RGD-MCLs) was able to facilitate cell growth, cell sheet constr...