نتایج جستجو برای: cell labeling
تعداد نتایج: 1721447 فیلتر نتایج به سال:
Current method to magnetically label cells using ferumoxides (Fe)-protamine (Pro) sulfate (FePro) is based on generating FePro complexes in a serum free media that are then incubated overnight with cells for the efficient labeling. However, this labeling technique requires long (>12-16 hours) incubation time and uses relatively high dose of Pro (5-6 microg/ml) that makes large extracellular FeP...
Abstract Cell-free protein synthesis (CFPS) enables the development of antibody conjugates, such as fluorophore conjugates and antibody-drug (ADCs), in a rapid straightforward manner. In first part, we describe cell-free antibodies containing fluorescent non-canonical amino acids (ncaa) by using pre-charged tRNA. second ncaa an orthogonal system, followed site-specific conjugation dye DyLight 6...
A rapid and reproducible method for the preparation of autoradiographs from single-cell suspensions of human tumor cells, including melanoma and lung carcinoma, is described. Tritiated thymidine of high specific activity (6.0 Ci/mmole) was used so that autoradiographs could be developed and read at 24 hr after the sample was taken. Samples were prepared in duplicate, and a Hypaque-Ficoll densit...
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