Objective(s) The use of co-culture cells as well as extra cellular matrix are among those strategies that have been employed to direct mesenchymal stem cell (MSC) bone differentiation in culture. In this regard, there is no study considering the effects of Matrigel on mesenchymal stem cell (MSC) in vitro bone differentiation. This was the subject of the present study. Materials and Methods ...

Background During the chronic myelogenous leukemia (CML) the Bcr-Abl oncoprotein is produced, which leads to unregulated cell proliferation. CML is treated with one of several targeting therapies such as imatinib, (formerly STI-571 [Glivec; Novartis, Switzerland]) a selective inhibitor that blocks tyrosin kinase activity of the Bcr-Abl oncoprotein. Apart from the second generation Bcr-Abl inhib...

BACKGROUND Here we describe a novel cultivation method, called EnBasetrade mark, or enzyme-based-substrate-delivery, for the growth of microorganisms in millilitre and sub-millilitre scale which yields 5 to 20 times higher cell densities compared to standard methods. The novel method can be directly applied in microwell plates and shake flasks without any requirements for additional sensors or ...

In general, morphological, physiological, and biochemical properties of fungal cells are drastically different among solid-state (SSC), liquid-surface (LSC), and submerged cultivation (SmC) systems. In addition to these traditional cultivation systems, a unique liquid-surface cultivation [liquid-surface immobilization (LSI)] system and interface cultivation systems [extractive liquid-surface im...

The majority of biotechnologically relevant metabolites do not impart a conspicuous phenotype to the producing cell. Consequently, the analysis of microbial metabolite production is still dominated by bulk techniques, which may obscure significant variation at the single-cell level. In this study, we have applied the recently developed Lrp-biosensor for monitoring of amino acid production in si...

Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, ...

Sponges host complex microbial communities of recognized ecological and biotechnological importance. Extensive cultivation efforts have been made to isolate sponge bacteria, but most still elude cultivation. To identify the bottlenecks of sponge bacterial cultivation, we combined high-throughput 16S rRNA gene sequencing with a variety of cultivation media and incubation conditions. We aimed to ...

Co-cultivation of thymus and spleen cells of Fisher and Lewis rats with lethally irradiated MT-2 cells harboring human T-cell leukemia virus type I (HTLV-I) resulted in the establishment of lymphoid cell lines, FIRT-1, FIRS-1, LERT-1, and LERS-1, respectively. Cells of these cell lines had rat T-cell characters as demonstrated by the positive reaction to monoclonal antibodies (MAbs) to rat T ce...

Background Increasing cell specific productivity is still an important challenge in today’s production of biopharmaceuticals in mammalian cell cultures. During the last two decades various efforts were made to achieve higher yields, e.g. by optimization of process strategies, media development or cell line and metabolic engineering. Nevertheless, some challenges remain for efficient and applica...