cell clamp

نتایج جستجو برای: cell clamp

تعداد نتایج: 1696485 فیلتر نتایج به سال:

Integration of autopatching with automated pipette and cell detection in vitro.

Journal: :Journal of neurophysiology 2016

Qiuyu Wu 吴秋雨 Ilya Kolb Brendan M Callahan Zhaolun Su William Stoy Suhasa B Kodandaramaiah Rachael Neve Hongkui Zeng Edward S Boyden Craig R Forest Alexander A Chubykin

Patch clamp is the main technique for measuring electrical properties of individual cells. Since its discovery in 1976 by Neher and Sakmann, patch clamp has been instrumental in broadening our understanding of the fundamental properties of ion channels and synapses in neurons. The conventional patch-clamp method requires manual, precise positioning of a glass micropipette against the cell membr...

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The TCF C-clamp DNA binding domain expands the Wnt transcriptome via alternative target recognition

2014

Nate P. Hoverter Michael D. Zeller Miriam M. McQuade Angela Garibaldi Anke Busch Elizabeth M. Selwan Klemens J. Hertel Pierre Baldi Marian L. Waterman

LEF/TCFs direct the final step in Wnt/β-catenin signalling by recruiting β-catenin to genes for activation of transcription. Ancient, non-vertebrate TCFs contain two DNA binding domains, a High Mobility Group box for recognition of the Wnt Response Element (WRE; 5'-CTTTGWWS-3') and the C-clamp domain for recognition of the GC-rich Helper motif (5'-RCCGCC-3'). Two vertebrate TCFs (TCF-1/TCF7 and...

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