Aqueous dispersions of hydrophobic high-molar-mass cellulose-graft-polystyrene (Cel-g-PS) copolymers were prepared by nanoprecipitation from dioxane solutions using sodium dodecyl sulfate as a stabilizer. The size and structure of formed Cel-g-PS nanoparticles were studied by scattering techniques (static and dynamic light scattering, SAXS) and transmission electron microscopy. The scattering a...

We developed a xenograft model of human Chronic Eosinophilic Leukemia (CEL) to study disease progression and remission-induction under therapy with tyrosine kinase inhibitors using imatinib and nilotinib as examples. The FIP1L1/PDGFRA+ human CEL cell lineEOL-1 was injected intravenously into scid mice, and MR imaging and FACS analysis of mouse blood samples were performed to monitor disease dev...

mRNA capping requires the sequential action of three enzymatic activities: RNA triphosphatase, guanylyl-transferase, and methyltransferase. Here we characterize a gene (CEL-1) believed to encode the C. elegans capping enzyme. CEL-1 has a C-terminal domain containing motifs found in yeast and vaccinia virus capping enzyme guanylyltransferases. The N-terminal domain of CEL-1 has RNA triphosphatas...

BACKGROUND Recently in China, HCV 6a infection has shown a fast increase among patients and blood donors, possibly due to IDU linked transmission. METHODOLOGY/FINDINGS We recruited 210 drug users in Shanwei city, Guangdong province. Among them, HCV RNA was detected in 150 (71.4%), both E1 and NS5B genes were sequenced in 136, and 6a genotyped in 70. Of the 6a sequences, most were grouped into...

Diets that are high in dietary fiber are reported to have substantial health benefits. We sought to compare the metabolic effects of 3 types of dietary fibers -- sugarcane fiber (SCF), psyllium (PSY), and cellulose (CEL) -- on body weight, carbohydrate metabolism, and stomach ghrelin gene expression in a high-fat diet-fed mouse model. Thirty-six male mice (C57BL/6) were randomly divided into 4 ...

A novel stability-indicating reverse phase high performance liquid chromatography method was developed and validated for the simultaneous determination of Celecoxib (CEL) and Diacerein (DIN) and its impurities in capsule dosage form. The method was developed using L1 column with gradient using the mobile phase consist of Solution A (pH = 2.3 buffer) and Solution B (methanol and acetonitrile; 50...

The genome sequence of the original human herpesvirus 6A (HHV-6A) (GS isolate) has never been determined. DNA from a very low passage (<5 times) HHV-6A GS stock was isolated and sequenced, making it the first HHV-6A of U.S. origin to be fully sequenced. Such a sequence will be helpful for comparative analysis with other HHV-6A strains, including the U1102 strain isolated from Uganda.

Downlo ivated oncogenes are the dominant drivers of malignant progression in human cancer, yet little is known how the transformation from proto-oncogene to activated oncogene drives the expression of transd phenotypes. An isogenic model of HER-2–mediated transformation of human mammary epithelial as used along with HER-2–amplified human breast cancers to investigate how HER-2 activation alters...

TGFBR1*6A is a common hypomorphic variant of the type I transforming growth factor (TGF)-beta receptor (TGFBR1), which transduces TGF-beta growth inhibitory signals less effectively than TGFBR1. Recent studies suggest that TGFBR1*6A confers a selective growth advantage to both normal appearing and cancerous epithelial cells in the presence of TGF-beta. We have previously shown that TGFBR1*6A is...

Ly-6A/Stem cell antigen-1 (Ly-6A/Sca-1) is a glycosylphosphatidylinositol-anchored protein expressed on many cell types including hematopoietic stem cells (HSCs) and early lymphoid-specific progenitors. Ly-6A/Sca-1 is expressed on CD4+ T cells and plays a role in regulating cellular responses to foreign antigens. The role of Ly-6A/Sca-1 in primary antibody responses has not been defined. To inv...