The specificity of chitinase C-1 of Streptomyces griseus HUT 6037 for the hydrolysis of the beta-1,4-glycosidic linkages in partially acetylated chitosan is different from that of other microbial chitinases. In order to study the primary structure of this unique chitinase, the chiC gene specifying chitinase C-1 was cloned and its nucleotide sequence was determined. The gene encodes a polypeptid...

Sucrose hydrolysis reaction, catalysed by invertase under microwave irradiation was carried out in aqueous, isopropanol + water and polyethylene glycol + water system. The effect of conventional and microwave heating on the rate of hydrolysis was studied under a range of physical exposure conditions. The catalytic activity of invertase in aqueous and organic solvents were measured at different ...

We report the synthesis and characterization of new Ni(x)Ru(1-x) (x = 0.56-0.74) alloy nanoparticles (NPs) and their catalytic activity for hydrogen release in the ammonia borane hydrolysis process. The alloy NPs were obtained by wet-chemistry method using a rapid lithium triethylborohydride reduction of Ni(2+) and Ru(3+) precursors in oleylamine. The nature of each alloy sample was fully chara...

Strain is eliminated as a factor in hydrolysis of the scissile peptide bond by human immunodeficiency virus (HIV)-1 and simian immunodeficiency virus (SIV), based on the first eight complexes of products of hydrolysis with the enzymes. The carboxyl group generated at the scissile bond interacts with both catalytic aspartic acids. The structures directly suggest the interactions of the gemdiol i...

Homocysteinylation of lysine residues by homocysteine thiolactone (HCTL), a reactive homocysteine metabolite, results in protein aggregation and malfunction, and is a well-known risk factor for cardiovascular, autoimmune and neurological diseases. Human plasma paraoxonase-1 (PON1) and bleomycin hydrolase (Blmh) have been reported as the physiological HCTL detoxifying enzymes. However, the catal...

Dilute FeCl3 and Fe(NO3)3 solutions degraded parathion to paraoxon and p-nitrophenol. Initial hydrolysis products of iron appeared to have the greatest catalytic activity which decreased as these hydrolysis products aged. The Fe3+ ion was less catalytically active than its hydrolysis products for parathion degradation. pH was not a factor in parathion degradation in this study. AlCl3 solutions ...

Three different types of biomass sourced from forestry waste (eucalyptus residues), agricultural (wheat straw), and energy crop (miscanthus) were used as starting materials to produce hemicellulosic sugars, furans (furfural hydroxymethylfurfural), oligosaccharides. A two-step hybrid process was implemented; first autohydrolysed without any additive extract hemicelluloses dissolve it in water. T...

Understanding the dynamics of the key pectinase, polygalacturonase, and improving its thermotolerance and catalytic efficiency are of importance for the cost-competitive bioconversion of pectic materials. By combining structure analysis and molecular dynamics (MD) simulations, eight mutagenesis sites having the potential to form cation-π interactions were identified in the widely used fungal en...

BACKGROUND YqeH, a circularly permuted GTPase (cpGTPase), which is conserved across bacteria and eukaryotes including humans is important for the maturation of small (30S) ribosomal subunit in Bacillus subtilis. Recently, we have shown that it binds 30S in a GTP/GDP dependent fashion. However, the catalytic machinery employed to hydrolyze GTP is not recognized for any of the cpGTPases, includin...

F(1) ATPase, a rotary motor comprised of a central stalk (gamma subunit) enclosed by three alpha and beta subunits alternately arranged in a hexamer, features highly cooperative binding and hydrolysis of ATP. Despite steady progress in biophysical, biochemical, and computational studies of this fascinating motor, the structural basis for cooperative ATPase involving its three catalytic sites re...