The salt of milk constitutes a small part of milk (8-9 g.L(-1)); this fraction contains calcium, magnesium, sodium and potassium for the main cations and inorganic phosphate, citrate and chloride for the main anions. In milk, these ions are more or less associated between themselves and with proteins. Depending on the type of ion, they are diffusible (cases of sodium, potassium and chloride) or...

Abstract The purpose of this study was to evaluate the quality mountain sheep milk obtained from animals kept on pastures: natural and organic. experimental procedure conducted under production conditions, during annual grazing two pastures in region Polish Carpathians: organic pasture certified for farming belonging National Park. This showed greater plant species diversity pasture, but its yi...

Casein has been widely used for the assay of proteolytic enzymes since Kunitz (1947) described his procedure for the measurement of the activity of trypsin and chymotrypsin. Several procedures have since been designed for the assay of plasmin, the most widely used being that of Remmert & Cohen (1949). These workers activated plasminogen with excess of streptokinase in the presence of casein and...

Methods b-Lactoglobulin, whole casein and its four different fractions, i.e. as1-, as2-, band -caseins, were isolated from raw CM and GM. Purified b-caseins were subjected to a mild proteolysis by plasmin which generated 3 peptides, i.e. f(1-28), f(29-107) and f(108-207/9). Synthetic peptides partially recovering the N-terminal f(29107) part of the caprine b-casein were also produced. Immunorea...

A two-step-cascade membrane separation by ultrafiltration was performed on caprine milk prepared under different temperature conditions to eliminate beta-lactoglobulin (β-Lg) from the whey fraction. Effects of temperature treatment and membrane pore size on the elimination of β-Lg and retention of alpha-lactalbumin (α-La) were examined to determine the optimum permeate fraction for production o...

Extracts of HeLa cell fractions were analyzed by DEAE- and phospho-cellulose chromatography for their range of cyclic AMP-dependent and -independent protein kinase activities phosphorylating histone and/or phosvitin; extractions were by phosphate buffered saline (soluble protein kinases) and the non-ionic detergent NP-40 (membrane-bound protein kinases). The soluble fraction contained (i) cycli...

Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients. The objective of this study was to characterize the anti-inflammatory properties of sodium caseinate (NaCAS), its enzyme hydrolysate (EH) and peptide-enriched fractions (5 kDa retentate [R], 1 kDaR and 1 kDa permeate [P]), both in vitro using a Caco-...

The objective of this study was to determine the effect of supplemental lysine (Lys), methionine (Met) or both amino acids added in the form of rumen-protected (RP) tablets with copolymer coating to a diet of dairy cows on yield and composition of milk and concentration of plasma amino acids (AA). The experiment was carried out on four high-yielding lactating Holstein cows with average milk pro...