Virus-like particles (VLPs) serve as excellent model systems to identify the pathways of virus assembly. To gain insights into the assembly mechanisms of the Physalis mottle tymovirus (PhMV), six interfacial residues, identified based on the crystal structure of the native and recombinant capsids, were targeted for mutagenesis. The Q37E, Y67A, R68Q, D83A, I123A, and S145A mutants of the PhMV re...

We report here the complete genome sequence of isolate T32 of parietaria mottle virus (PMoV) infecting tomato plants in Turin, Italy, obtained by Sanger sequencing. T32 shares 90.48 to 96.69% nucleotide identity with other two PoMV isolates, CR8 and Pe1, respectively, whose complete genome sequences are available.

Pepper mottle virus is a member of genus Potyvirus and infects many plants worldwide. Bell pepper (Capsicum annuum) leaves with symptoms were collected, after using RT-PCR amplification virus-specific primers for the coat protein, amplicons sequenced. Sequences revealed presence (PeMV) first time in Iran. Phylogenetic analysis protein sequences showed that Iranian isolates shared greatest pairw...

In sub-Saharan Africa, sweetpotato (Impomoea batatas L.) production is greatly constrained by sweetpotato virus disease (SPVD) complex. This study was conducted to assess the incidence of viruses in healthy-looking sweetpotato in Uganda and to optimise modern technologies for virus diagnosis. A collection of healthy-looking sweetpotato vines from central Uganda were serologically assayed for sw...

Carnation (Dianthus caryophylinus) is one of the most popular ornamental flowers worldwide. etched ring virus (CERV), belonging to Caulimovirus genus, second significant on carnation crops around world. CERV particles are isometric, with a diameter 47 nm, which contains double-stranded, circular DNA at length about 7932 kbp as genome. causes necrotic flecks, rings and line patterns leaves. Infe...

Senescence of carnation flowers is characterized by autocatalytic ethylene production from petals and subsequent wilting of the petals. Recent studies on the regulation of ethylene production and wilting in senescing carnation petals revealed that (1) petal senescence is triggered by ethylene evolved from the gynoecium during natural senescence, (2) ethylene production in the gynoecium is induc...

We have determined the nucleotide sequence of the region of red clover mottle virus (RCMV) bottom component RNA which encodes the RCMV equivalent of the cowpea mosaic virus (CPMV) 24K protease. From the alignment of the deduced amino acid sequence of the RCMV 24K protein with that of the homologous protein from CPMV, we speculate on the relative importance of the various amino acid residues whi...

During routine graft indexing of Rubus clones at the USDA-ARS Horticultural Crops Research Laboratory in Corvallis, Oregon, a ‘Glen Clova’ plant that originated in Scotland induced severe symptoms including stunting and apical necrosis when grafted on ‘Munger’ black raspberry (Rubus occidentalis). Cloning of viral dsRNA obtained from the plant and sequence analysis indicated the presence of a n...

A graft-transmissible disease of cherry trees herein referred to as mottle leaf is known to have existed in different sections of the Pacific Northwest for the last several years. In 1917 D. F. Fisher photographed ^ cherry leaf specimens collected near Monitor, Wash., which exhibited symptoms similar to mottle leaf. Zeller (8) * reported having observed the disease in 1920 on Napoleon (Royal An...