We have studied the transport of soluble cargo molecules by inhibiting specific transport steps to and from the Golgi apparatus. Inhibition of export from the Golgi via coexpression of a dominant-negative GTP-restricted ARF1 mutant (Q71L) inhibits the secretion of alpha-amylase and simultaneously induces the secretion of the vacuolar protein phytepsin to the culture medium. By contrast, specifi...

Highly conserved EB1 family proteins bind to the growing ends of microtubules, recruit multiple cargo proteins, and are critical for making dynamic microtubules in vivo. However, it is unclear how these master regulators of microtubule plus ends promote microtubule dynamics. In this paper, we identify a novel EB1 cargo protein, Sentin. Sentin depletion in Drosophila melanogaster S2 cells, simil...

Proteins containing a C-terminal thioester are important intermediates in semisynthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. Here we report a simple strategy, utilizing sortase A, for routine preparation of recombinant proteins containing a C-terminal (α)thioester. We used our method to prepare two different ...

This paper establishes an approach to forecast air cargo demand related to terminal capacity expansion. To balance capacity and demand, it is required to forecast the future demand based on optimistic and pessimistic projections to decide when and how much, the airport should expand the capacity. System dynamics simulation model can provide reliable forecast and generate scenarios to test alter...

International cargo transportation is majorly dependent on marine transport, which moves 80% of the international cargo. With increase in vessel size and same yard area available for container transportation, it important to study processes understand efficiency operations process. This research paper aims review import process cycle through lean six sigma concepts. The evaluated from perspecti...

The purpose of the work is to improve technology optimize functioning container terminal. An important role in terminals played by yards, together with personnel who handle loading and unloading operations containers their sorting. working same, but there are many new devices that can be used for sorting accounting containers. methodology. interaction between workers, technical means transport ...

tors by the specific names used in their original descriptions. These receptors are generally large (90–130 kDa) Philadelphia, Pennsylvania 19104-6148 acidic proteins sharing 15%–25% sequence identity within a given organism. They all have an N-terminal RanGTP-binding domain, a C-terminal cargo-binding Eukaryotic cells are equipped with a machinery charged domain, and the capacity to bind compo...

Marine terminal operations are complex, and evaluating changes is best done using a simulation tool that captures the dynamics and interactions of the system. We have developed a flexible and robust discrete-event simulation of a marine terminal that handles liquid cargo. We used this simulation to investigate proposed changes generated by a Six Sigma project to reduce congestion at the termina...

The container terminal is a convergence point in the cargo flow, being a focal point for multiple supply chains. The operations performed in a container terminal involve one of the most complex environments within the transport industry. A container terminal plays a critical role in international shipping and is under pressure to cope with increasing container traffic. This paper presents a mul...

This paper intends to assess the potential impacts of future SLR on operability berthing structures and estimate in monetary terms adaptation costs it. To do this, three scenarios are considered, two corresponding last assessment report IPCC (RCP4.5 RCP8.5) other being a high-end scenario (HES), with low probability occurrence but physically possible. The research is focused case study Tangier-...