نتایج جستجو برای: carbon catabolite repression

تعداد نتایج: 305443  

Journal: :Applied and environmental microbiology 2012
Jiaofang Huang Elisabeth Sonnleitner Bin Ren Yuquan Xu Dieter Haas

In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition sequence that was interrupted by the AUG translation initiation codon.

Journal: :Microbial Cell Factories 2008
Karla Martínez Ramón de Anda Georgina Hernández Adelfo Escalante Guillermo Gosset Octavio T Ramírez Francisco G Bolívar

BACKGROUND Escherichia coli strains lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) are capable of coutilizing glucose and other carbon sources due to the absence of catabolite repression by glucose. In these strains, the lack of this important regulatory and transport system allows the coexistence of glycolytic and gluconeogenic pathways. Strains lacking PTS have ...

Journal: :Journal of bacteriology 1967
B Tyler W F Loomis B Magasanik

Severe transient repression of constitutive or induced beta-galactosidase synthesis occurs upon the addition of glucose to cells of Escherichia coli growing on glycerol, succinic acid, or lactic acid. Only mutants particularily well adapted to growth on glucose exhibit this phenomenon when transferred to a glucose-containing medium. No change in ribonucleic acid (RNA) metabolism was observed du...

Journal: :Microbiology 1999
S Hohmann J Winderickx J H de Winde D Valckx P Cobbaert K Luyten C de Meirsman J Ramos J M Thevelein

In the yeast Saccharomyces cerevisiae, glucose or fructose represses the expression of a large number of genes. The phosphorylation of glucose or fructose is catalysed by hexokinase PI (Hxk1), hexokinase PII (Hxk2) and a specific glucokinase (Glk1). The authors have shown previously that either Hxk1 or Hxk2 is sufficient for a rapid, sugar-induced disappearance of catabolite-repressible mRNAs (...

Journal: :Journal of bacteriology 2009
Catalina Arango Pinedo Daniel J Gage

The HPrK kinase/phosphatase is a common component of the phosphotransferase system (PTS) of gram-positive bacteria and regulates catabolite repression through phosphorylation/dephosphorylation of its substrate, the PTS protein HPr, at a conserved serine residue. Phosphorylation of HPr by HPrK also affects additional phosphorylation of HPr by the PTS enzyme EI at a conserved histidine residue. S...

Journal: :Nucleic acids research 1990
Y Miwa Y Fujita

The mechanism underlying catabolite repression in Bacillus species remains unsolved. The gluconate (gnt) operon of Bacillus subtilis is one of the catabolic operons which is under catabolite repression. To identify the cis sequence involved in catabolite repression of the gnt operon, we performed deletion analysis of a DNA fragment carrying the gnt promoter and the gntR gene, which had been clo...

Journal: :The Journal of biological chemistry 1999
J Stolz N Sauer

The product of the FEN2 gene of Saccharomyces cerevisiae has previously been described as a protein conferring sensitivity to the antifungal agent fenpropimorph. Fen2p was postulated to act as a common regulator of carbon and nitrogen catabolite repression and of amino acid and ergosterol biosynthesis. In this paper, we present experimental evidence characterizing Fen2p as a plasma membrane-loc...

Journal: :Journal of bacteriology 1992
T Rygus W Hillen

We characterized catabolite repression of the genes encoding xylose utilization in Bacillus megaterium. A transcriptional fusion of xylA encoding xylose isomerase to the spoVG-lacZ indicator gene on a plasmid with a temperature-sensitive origin of replication was constructed and efficiently used for single-copy replacement cloning in the B. megaterium chromosome starting from a single transform...

Journal: :Fermentation 2021

Wine strains of Saccharomyces cerevisiae have to adapt their metabolism the changing conditions during biotechnological use, from aerobic growth in sucrose-rich molasses for biomass propagation anaerobic fermentation monosaccharides grape juice winemaking. Yeast molecular mechanisms that favor use preferred carbon and nitrogen sources achieve such adaptation. By using specific inhibitors, it wa...

Journal: :The Journal of antimicrobial chemotherapy 2007
A Bizzini J M Entenza P Moreillon

OBJECTIVES Antibiotic tolerance is a phenomenon allowing bacteria to withstand drug-induced killing. Here, we studied a penicillin-tolerant mutant of Streptococcus gordonii (Tol1), which was shown to be deregulated in the expression of the arginine deiminase operon (arc). arc was not directly responsible for tolerance, but is controlled by the global regulator CcpA. Therefore, we sought whether...

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