Sequential fractionation of extractive-free maize stems was carried out using two mild alkaline extractions (0.5 and 2 M NaOH, 20°C, 24h) before and after endoglucanase treatment. This procedure provided two lignin-carbohydrate fractions (LC1 and LC2) recovered after each alkali treatment. LC1 and LC2 contained 39% and 8% of the total lignin amount, respectively. These two fractions contained s...

A rise of the polysaccharide associated with serum proteins has been noted in malignancy and in several other conditions (7). Seibert, Pfaff, and Seibert (4) determined the carbohydrate content of normal plasma protein fractions isolated by the low temperature ethanolic procedure of Cohn et al. (1) and foundconsiderablecarbohydrateto be associated with all globulin fractions. It would be of int...

Concentrations and chemical composition of carbohydrates in dissolved organic carbon (DOC) and in extracellular polymeric substances (EPS) from brines, ice cores, gap layers in sea ice and associated surface waters were determined during 2004 and 2006 in the Weddell Sea, Antarctica. High levels of spatial heterogeneity were a common feature in these habitats, with DOC concentrations ranging fro...

1. The effects of Ca(2+) ions and the nutritional state of the animals on the distribution of soluble protein between the medium, cytoplasmic and particulate fractions after incubation of rat-liver slices have been studied. 2. The Ca(2+)ions decreased the leakage of cytoplasmic protein into the medium and increased the loss of protein from the particles; the effects were more marked with livers...

Soybean hull peroxidase (SBP, E.C. 1.11.1.7), an anionic glycoprotein, was found to contain 18.2% carbohydrate with the average composition: 2 mol GlcNAc, 3.3 mol Man, 0.9 mol Fuc, and 0.7 mol Xyl. The oligosaccharides of SBP, after release with glycopeptidase A, were investigated by a combination of high pH anion exchange chromatography with pulsed amperometric detection, methylation analysis ...

1. Gel filtration of the water-soluble radioactive mucus produced three radioactive fractions, fraction A excluded on Sepharose 4B, fraction B included on Sepharose 4B but excluded on Sephadex G-200, and fraction C included on Sephadex G-200. 2. The specific radioactivities of fractions A and B were the same, with fraction C a little lower, whether the material was labelled with (14)C-labelled ...

A sequential chemical extraction with a defined series of eluotropic organic solvents an increasing polarity (trichloromethane < ethyl acetate acetone acetonitrile n-propanol methanol) was performed on peat-bog humic acid. Six fractions were obtained and subjected to physicochemical characterization utilizing methods structural compositional analysis. Advanced spectroscopic techniques such a...

The trypsinized cell walls of Group C hemolytic streptococci are composed of the specific carbohydrate antigen and a mucopeptide matrix. Certain phage resistant strains of Group C streptococci, isolated from organisms which survive exposure to Group C bacteriophage also possess carbohydrate and mucopeptide fractions, but the carbohydrate gives a precipitin reaction with Group A-variant antiseru...