Successful total RNA (totRNA) extraction is a prerequisite for a successful real-time PCR. In the present work we compared one manual and one automated totRNA extraction method for detection of Plum pox potyvirus (PPV) in leaves of different Prunus sp. using developed real-time RT-PCR assay. Advantages and disadvantages of compared methods are described in the view of sensitivity, reproducibili...

Mayr A. & Mayr B. 1999. A new concept in prophylaxis and therapy: paramunization by poxvirus inducers. Pesquisa Veterinária Brasileira 19(3/4):91-98. Lehrstuhl für Mikrobiologie und Seuchenlehre der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München, Veterinärstraße 13, D-80539 München, Germany. The so-called primitive, innate or paraspecific immune system is the phylogeneticall...

The Pax gene Pox meso (Poxm) was the first and so far only gene whose initial expression was shown to occur specifically in the anlage of the somatic mesoderm, yet its role in somatic myogenesis remained unknown. Here we show that it is one of the crucial genes regulating the development of the larval body wall muscles in Drosophila. It has two distinct functions expressed during different phas...

In one proposed use of digital watermarks, the owner of a document D sells slightly diierent documents, D 1 ; D 2 ; : : : to each buyer; if a buyer posts his/her document D i to the web, the owner can identify the source of the leak. More general attacks are however possible in which k buyers create some composite document D ; the goal of the owner is to identify at least one of the conspirator...

ABSTRACT Plum pox potyvirus (PPV) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. Monoclonal antibodies specific for the two major groups of isolates, represented by the D and M serotypes of the virus, have been obtained. Polymerase chain reaction (PCR)-based assays allowing the direct detection and differentiation of PPV isolates h...

Sharka is a disease of fruiting, ornamental and wild Prunus species of great socio-economic relevance that has spread to most Prunusgrowing nations. It is caused by Plum pox virus (PPV), a member of the genus Potyvirus. In recent years, there have been notable advances in our understanding of genome organization and expression, functions of gene products, and pathogenicity and host range determ...

Rugby football players know that skin infections may be transmitted by bodily contact. Forwards in particular are likely to develop facial lesions from face-to-face contact in scrums. Players refer to the skin lesions by various names including "scrum-pox," "prop-pox," and "impetigo." Some of these infections are undoubtedly bacterial and there have been cases of herpes simplex-1 and vaccinia4 ...