1. A liver canalicular plasma-membrane fraction enriched 115-155-fold in five marker enzymes relative to the tissue homogenate was obtained by sonication of liver plasma membranes followed by fractionation in iso-osmotic Nycodenz gradients. 2. Two lateral-plasma membrane fractions were also collected by this procedure; the lighter-density fraction was still associated with canalicular membranes...

The bile salt export pump (BSEP, ABCB11) couples ATP hydrolysis with transport of bile acids into the bile canaliculus of hepatocytes. Its localization in the apical canalicular membrane is physiologically regulated by the demand to secrete biliary components. To gain insight into how such localization is regulated, we studied the intracellular trafficking of BSEP tagged with yellow fluorescent...

Viral infection is a common cause of acquired obstruction of the lacrimal canalicular system. A series of 20 patients with canalicular obstruction attributable to infection with herpes simplex is reported, and 1 case is described in detail.

INTRODUCTION Ocular injuries commonly involve the lacrimal system. CASE A 14-year-old male had an eyelid injury with a canalicular tear. An angiocatheter tube was used as a stent for canalicular reconstruction. The focus of this report is on the method used, as well as the material for the repair in a rural hospital. CONCLUSION Angiocatheter tube can be a suitable alternative to silicone tu...

The bile salt export pump (BSEP, ABCB11) couples ATP hydrolysis with transport of bile acids into the bile canaliculus of hepatocytes. Its localization in the apical canalicular membrane is physiologically regulated by the demand to secrete biliary components. To gain insight into how such localization is regulated, we studied the intracellular trafficking of BSEP tagged with yellow fluorescent...

To better understand the regulation of biliary phospholipid and cholesterol excretion, canalicular membranes were isolated from the livers of C57BL/6J mice and abundant proteins separated by SDS-PAGE and identified by matrix-assisted laser desorption/ionization mass spectrometry. A prominent protein revealed by this analysis was betaine homocysteine methyltransferase (BHMT). This enzyme catalyz...

OBJECTIVES To evaluate the surgical outcomes of endoscopic dacryocystorhinostomy followed by canalicular trephination and silicone stenting in patients with distal or common canalicular obstructions. METHODS The medical records of 29 patients (31 eyes) from January 2001 to December 2009 who underwent endoscopic dacryocystorhinostomy followed by canalicular trephination and silicone tube inser...

Purpose. The aim of this study is to propose a simple and efficient combination surgery for the management of dacryocystitis with canalicular obstruction. Methods. A retrospective noncomparative case series of dacryocystitis with canalicular obstruction has been studied. Twelve patients with dacryocystitis and canalicular obstruction underwent a conventional endoscopic endonasal dacryocystorhin...

words/characters: 226/1,782; Manuscript words/characters: 8,118/55,174; Figures (black and white): 3; Figures (color): 3; Tables: 1 Conflict of Interest: All authors have no conflicts of interest. Initial Date Submitted April 30, 2010; Date Revision Submitted July 9, 2010; Date Final Disposition Set August 24, 2010 Journal of Bone and Mineral Research © 2010 American Society for Bone and Minera...

As a woman, labor experience could be best memory, but it was also became trauma for them especially perineum laceration. Perineum laceration occurred during process naturally or instrumentally (episiotomy, extraction). caused discomfort and pain heacting process. The fearness affected mother psychology. To minimize the laceration, perineal massage prevent grade II incidence. determined applica...